血凝抑制技术(HI)在鸵鸟新城疫诊断中的应用

A. O. T. Carrasco, O. C. F. Neto, S. L. S. Lages, R. Sousa, A. B. Junior, A. A. Pinto
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引用次数: 2

摘要

近年来,大鼠(鸵鸟、鸸鹋和大鼠)的繁殖在世界范围内大幅扩大。血凝抑制(HI)试验被认为是标准的血清学试验,用于检测抗新城疫病病毒(NDV)的抗体。然而,在某些物种(包括鸵鸟)的血清样本中,HI测试往往会产生假阳性结果。在本研究中,高岭土被用于消除鸵鸟血清样品中的非特异性血凝抑制剂。27个未经高岭土处理的鸵鸟样品对测试有反应,滴度从2到64不等。然而,这些样品在高岭土处理后没有反应。不管用高岭土治疗,对照组在抗体滴度上没有表现出任何差异。将高岭土用于ND的常规血清学诊断中,不仅减轻了血清样品与禽类红细胞的吸附,而且有助于减少供体禽类红细胞的维持。DOI: http://dx.doi.org/10.17525/vrr.v13i1 - 2.3
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ADAPTATION OF HEMAGGLUTINATION INHIBITION TECHNIQUE (HI) FOR THE DIAGNOSIS OF NEWCASTLE DISEASE IN OSTRICHES (Struthio camellus)
The  breeding  of  ratites  (ostriches,  emus  and  rheas)  has  expanded  considerably  all over the world in recent years. The hemagglutination inhibition (HI) test is considered to be the  standard  serology  test,  for  detection  of  antibodies  against  Newcastle  Disease  Virus (NDV).  However,  HI  test  tends  to  give  false  positive  results  in  serum  samples  of  some species,  including  ostriches.  In  this  study,  kaolin  was  used  to  eliminate  the  nonspecific hemagglutination  inhibitors  in  ostrich  serum  samples.  Twenty-seven  non-kaolin-treated ostrich  samples  reacted  to  the  test  and  the  titres  ranged  from  2  to  64.  However,  these samples  did  not  react  when  they  were  kaolin-treated.  The  controls  did  not  show  any variance on antibodies titres, regardless of treatment with kaolin. The use of kaolin in the routine  serodiagnosis  of  ND  not  only  relieves  adsorption  of  serum  samples  with  avian erythrocytes,  but  also  contributes  to  the  reduction  in  the  maintenance  of  red  blood  cells donor birds. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.3
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