巴西巴西州牛痘样病毒的动物感染:1-西北地区

Bruno R. Simonetti, D. C. Abreu, J. Simonetti, M. C. Gonçalves, M. Silva, O. Barth, H. Schatzmayr
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引用次数: 11

摘要

在过去的九年里,巴西里约热内卢州西北地区一直在追踪奶牛的正痘病毒感染情况。我们实验室收到了动物卫生主管部门采集的50只动物的临床标本,用于实验室诊断,其中大多数是血液标本。通过所采用的至少一种实验室检测,已在46只动物中证实了正痘病毒感染:使用在该地区具有分子特征和分离的牛痘样菌株作为抗原的中和抗体检测;rt - pcr测试;电镜观察;在Vero细胞系中分离病毒获得的病毒株经电镜和逆转录聚合酶链反应(RT-PCR)鉴定为牛痘样病毒株,证实了这些病毒株在自然界的循环。在该区域还观察到与受感染动物有关的人间病例,其特点是在巴西里约热内卢州由牛痘样毒株引起的人畜共患病正在扩大。DOI: http://dx.doi.org/10.17525/vrr.v12i1 - 2.10
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ANIMAL INFECTIONS BY VACCINIA-LIKE VIRUSES IN THE STATE OF RIO DE JANEIRO: 1- NORTHWESTERN REGION
Orthopoxvirus infections in dairy cattle have been followed in the northwestern region of the state of Rio de Janeiro for the last nine years. Clinical specimens from 50 animals collected by Animal Health authorities have been received in our laboratory for laboratory diagnosis, most of them blood specimens. Orthopoxvirus infections have been confirmed in 46 animals by at least one of the laboratory tests employed: neutralizing antibody detection using as antigen a vaccinia-like strain molecularly characterized and isolated in the area; RT-PCR test; electron microscopy observation; and virus isolation in a Vero cell line. The virus strains obtained were identified as vaccinia-like by electron microscopy and reverse transcriptase polymerase chain reaction (RT-PCR), confirming the circulation of these strains in nature. Human cases associated with the infected animals have been also observed in the region, characterizing an expanding zoonosis caused by vaccinia-like strains in the state of Rio de Janeiro. DOI: http://dx.doi.org/10.17525/vrr.v12i1-2.10
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