比例平均居中动力学谱法光学探针同时测定抗坏血酸和半胱氨酸

IF 0.7 Q4 CHEMISTRY, ANALYTICAL

Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI:10.17721/moca.2019.163-170

A. Vishnikin, Yuliia V. Miekh, Y. Bazel, M. Al-Shwaiyat, G. O. Petrushina

{"title":"比例平均居中动力学谱法光学探针同时测定抗坏血酸和半胱氨酸","authors":"A. Vishnikin, Yuliia V. Miekh, Y. Bazel, M. Al-Shwaiyat, G. O. Petrushina","doi":"10.17721/moca.2019.163-170","DOIUrl":null,"url":null,"abstract":"A procedure for simultaneous kinetic analysis of a binary mixture of ascorbic acid (Asc) and cysteine (Cys) was developed using the method of mean centering of ratio kinetic profiles. The method is based on the difference in the rate of reactions of Asc and Cys with the complex of iron(III) with o-phenanthroline at pH 7.05. The use of an optical probe simplifies the carrying out of kinetic measurements, allows to obtain highly reproducible (Sr=0.01-0.02) results, and significantly reduces the time of analysis. The method of mean centering of ratio kinetic profiles is superior to the H-Point standard addition method, since it does not require such a long measurement time and there is no restriction on the constancy of absorbance of one of the components. Asc and Cys can be determined in the concentration range from 1 to 10 mg/L. The method was successfully used to determine Asc and Cys in dietary supplements.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Simultaneous Kinetic Spectrophotometric Determination Of Ascorbic Acid And Cysteine With An Optical Probe By Mean Centering Of Ratio Kinetic Profiles Method\",\"authors\":\"A. Vishnikin, Yuliia V. Miekh, Y. Bazel, M. Al-Shwaiyat, G. O. Petrushina\",\"doi\":\"10.17721/moca.2019.163-170\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A procedure for simultaneous kinetic analysis of a binary mixture of ascorbic acid (Asc) and cysteine (Cys) was developed using the method of mean centering of ratio kinetic profiles. The method is based on the difference in the rate of reactions of Asc and Cys with the complex of iron(III) with o-phenanthroline at pH 7.05. The use of an optical probe simplifies the carrying out of kinetic measurements, allows to obtain highly reproducible (Sr=0.01-0.02) results, and significantly reduces the time of analysis. The method of mean centering of ratio kinetic profiles is superior to the H-Point standard addition method, since it does not require such a long measurement time and there is no restriction on the constancy of absorbance of one of the components. Asc and Cys can be determined in the concentration range from 1 to 10 mg/L. The method was successfully used to determine Asc and Cys in dietary supplements.\",\"PeriodicalId\":18626,\"journal\":{\"name\":\"Methods and Objects of Chemical Analysis\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Methods and Objects of Chemical Analysis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17721/moca.2019.163-170\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and Objects of Chemical Analysis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17721/moca.2019.163-170","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

摘要

建立了一种同时分析抗坏血酸(Asc)和半胱氨酸(Cys)二元混合物的动力学方法。该方法是基于Asc和Cys与铁(III)与邻菲罗啉配合物在pH为7.05时反应速率的差异。光学探针的使用简化了动力学测量的进行，允许获得高度可重复性(Sr=0.01-0.02)的结果，并显着减少了分析时间。比值动力学剖面平均定心法优于h点标准加法法，因为它不需要很长的测量时间，并且不受其中一组分吸光度常数的限制。Asc和Cys可在1 ~ 10mg /L的浓度范围内测定。该方法可用于膳食补充剂中氨基酸和胱氨酸的测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Simultaneous Kinetic Spectrophotometric Determination Of Ascorbic Acid And Cysteine With An Optical Probe By Mean Centering Of Ratio Kinetic Profiles Method

A procedure for simultaneous kinetic analysis of a binary mixture of ascorbic acid (Asc) and cysteine (Cys) was developed using the method of mean centering of ratio kinetic profiles. The method is based on the difference in the rate of reactions of Asc and Cys with the complex of iron(III) with o-phenanthroline at pH 7.05. The use of an optical probe simplifies the carrying out of kinetic measurements, allows to obtain highly reproducible (Sr=0.01-0.02) results, and significantly reduces the time of analysis. The method of mean centering of ratio kinetic profiles is superior to the H-Point standard addition method, since it does not require such a long measurement time and there is no restriction on the constancy of absorbance of one of the components. Asc and Cys can be determined in the concentration range from 1 to 10 mg/L. The method was successfully used to determine Asc and Cys in dietary supplements.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Methods and Objects of Chemical Analysis CHEMISTRY, ANALYTICAL-

CiteScore

1.00

自引率

14.30%

发文量

期刊介绍： The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.