P. Silva, I. Medina, Jorge Luiz Marx Young, Vívian Tamaki
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引用次数: 1
摘要
Ananas comosus val . ananassoides (Baker) Coppens & f.l leal是塞拉多濒危生物群落的一种观赏凤梨科植物。因此,需要采用体外培养和微繁等方法来保护该物种。氮(N)主要以NO3-和/或NH4+的形式被植物吸收,并被同化成氨基酸。本研究的目的是评价该凤梨属植物的氮素同化作用。植株在改良MS培养基中分别添加15、30、60和90 mM的NO3-、NH4+或NH4NO3,培养7个月后转移到离体环境中进行驯化。NH4+处理植株死亡率高。在驯化过程中,施用30、60和90 mM N作为NH4NO3的植株生物量较高。在N同化方面,用NH4NO3栽培植株的GS和NR活性最高,而用NH4+栽培植株的GDH活性最高。因此,推荐用60 mM N作为NH4NO3对该品种进行离体和离体培养。
Nitrogen assimilation in the bromeliad Ananas comosus var. ananassoides (Baker) Coppens & F.Leal grown in vitro with different sources of inorganic nitrogen
ABSTRACT Ananas comosus var. ananassoides (Baker) Coppens & F.Leal is a native ornamental bromeliad of the endangered biome Cerrado. Therefore, approaches aimed at the preservation of this species, such as in vitro cultivation and micropropagation are needed. Nitrogen (N) is absorbed by plants, mainly as NO3- and/or NH4+, and assimilated into amino acids. The aim of this work was to evaluate the N assimilation in this bromeliad. Plants were grown in vitro for seven months in modified MS medium with 15, 30, 60, and 90 mM of N as NO3-, NH4+ or NH4NO3, and then transferred to ex vitro conditions for acclimatization. Plants grown with NH4+ had high mortality. During acclimatization plants cultivated with 30, 60, and 90 mM of N as NH4NO3 showed higher biomass. With regard to N assimilation, GS and NR showed the highest activity in plants cultivated with NH4NO3, whereas plants cultivated with NH4+ had the highest GDH activity. Consequently, in vitro and ex vitro cultivation of this species with 60 mM N as NH4NO3 is recommended.