明胶摩尔质量对海藻酸盐微粒包覆的影响

IF 1 4区化学 Q4 POLYMER SCIENCE

Polimeros-ciencia E Tecnologia Pub Date : 2021-01-01 DOI:10.1590/0104-1428.20210027

Joelma Correia Beraldo, Gislaine Ferreira Nogueira, A. S. Prata, C. Grosso

{"title":"明胶摩尔质量对海藻酸盐微粒包覆的影响","authors":"Joelma Correia Beraldo, Gislaine Ferreira Nogueira, A. S. Prata, C. Grosso","doi":"10.1590/0104-1428.20210027","DOIUrl":null,"url":null,"abstract":"The protein adsorption on the porous alginate microparticles was evaluated in regards to the coating ability and this protective effect during gastrointestinal assay. The coating was performed at suitable pH for optimized electrostatic interaction between protein and alginate. Concentrations of gelatin (HGE) and their hydrolysates (Collagel® (MGE) (> 10 kDa) and Fortigel® (LGE) (3 kDa)) from 1 to 10% (w/w) were tested. Higher protein adsorption was observed in the highest concentration of protein in solution and the amount adsorbed was inversely proportional to the degree of hydrolysis with 47.3, 41.4 and 29.3% of protein adsorbed when HGE, MGE and LGE were used, respectively. The particles that showed higher protein adsorption were submitted to gastrointestinal in vitro assay. In gastric simulation, 39.1, 41.8 and 49.0% of protein from HGE, MGE and LGE were solubilized while 81.3, 61.5 and 95.2% were solubilized after 5 h under enteric conditions.","PeriodicalId":20282,"journal":{"name":"Polimeros-ciencia E Tecnologia","volume":"1 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effect of molar weight of gelatin in the coating of alginate microparticles\",\"authors\":\"Joelma Correia Beraldo, Gislaine Ferreira Nogueira, A. S. Prata, C. Grosso\",\"doi\":\"10.1590/0104-1428.20210027\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The protein adsorption on the porous alginate microparticles was evaluated in regards to the coating ability and this protective effect during gastrointestinal assay. The coating was performed at suitable pH for optimized electrostatic interaction between protein and alginate. Concentrations of gelatin (HGE) and their hydrolysates (Collagel® (MGE) (> 10 kDa) and Fortigel® (LGE) (3 kDa)) from 1 to 10% (w/w) were tested. Higher protein adsorption was observed in the highest concentration of protein in solution and the amount adsorbed was inversely proportional to the degree of hydrolysis with 47.3, 41.4 and 29.3% of protein adsorbed when HGE, MGE and LGE were used, respectively. The particles that showed higher protein adsorption were submitted to gastrointestinal in vitro assay. In gastric simulation, 39.1, 41.8 and 49.0% of protein from HGE, MGE and LGE were solubilized while 81.3, 61.5 and 95.2% were solubilized after 5 h under enteric conditions.\",\"PeriodicalId\":20282,\"journal\":{\"name\":\"Polimeros-ciencia E Tecnologia\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polimeros-ciencia E Tecnologia\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1590/0104-1428.20210027\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"POLYMER SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polimeros-ciencia E Tecnologia","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1590/0104-1428.20210027","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}

引用次数: 1

摘要

在胃肠道试验中，对蛋白质在多孔藻酸盐微颗粒上的吸附进行了包衣能力和保护作用的评价。在合适的pH下进行涂膜，以优化蛋白质与海藻酸盐之间的静电相互作用。测定明胶(HGE)及其水解产物(Collagel®(MGE) (bbb10 kDa)和Fortigel®(LGE) (3 kDa)的浓度为1 ~ 10% (w/w)。在溶液中蛋白质的浓度最高时，蛋白质的吸附量较高，且吸附量与水解程度成反比，HGE、MGE和LGE的吸附量分别为47.3%、41.4%和29.3%。对蛋白质有较高吸附的颗粒进行了体外胃肠道实验。在胃模拟实验中，HGE、MGE和LGE蛋白的溶解率分别为39.1%、41.8%和49.0%，而在肠道条件下，5 h后的溶解率分别为81.3%、61.5%和95.2%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Effect of molar weight of gelatin in the coating of alginate microparticles

The protein adsorption on the porous alginate microparticles was evaluated in regards to the coating ability and this protective effect during gastrointestinal assay. The coating was performed at suitable pH for optimized electrostatic interaction between protein and alginate. Concentrations of gelatin (HGE) and their hydrolysates (Collagel® (MGE) (> 10 kDa) and Fortigel® (LGE) (3 kDa)) from 1 to 10% (w/w) were tested. Higher protein adsorption was observed in the highest concentration of protein in solution and the amount adsorbed was inversely proportional to the degree of hydrolysis with 47.3, 41.4 and 29.3% of protein adsorbed when HGE, MGE and LGE were used, respectively. The particles that showed higher protein adsorption were submitted to gastrointestinal in vitro assay. In gastric simulation, 39.1, 41.8 and 49.0% of protein from HGE, MGE and LGE were solubilized while 81.3, 61.5 and 95.2% were solubilized after 5 h under enteric conditions.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Polimeros-ciencia E Tecnologia 化学-高分子科学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Polímeros is a quarterly publication of the Associação Brasileira de Polímeros - ABPol (Brazilian Polymer Association), which publishes Review Articles, Original Articles and Short Communications, disclosing advances in the knowledge of Polymer Science and Technology.