M. Suleman, R. Faryal, Mian Muhammad Sufian, Y. Arshad
{"title":"巴基斯坦伊斯兰堡拉瓦尔品第2011年疫情期间登革热病毒感染的流行病学概况和实验室特征","authors":"M. Suleman, R. Faryal, Mian Muhammad Sufian, Y. Arshad","doi":"10.15406/JHVRV.2016.03.00099","DOIUrl":null,"url":null,"abstract":"A total of 215 serum samples of clinically suspected dengue patients who presented to the tertiary care hospitals over a period of July-November 2011 were analyzed for the detection of dengue NS1 antigen, dengue IgM and IgG antibodies and 180 (83.7%) samples were found positive by one or more than one testing parameters. Of the 180 samples, 98 (54.4%) were positive to NS1, 168 (93.3%) were positive to IgM and 93 (51.6%) cases were positive to IgG only. Similarly, 93 (51.6%) were positive to both NS1 and IgM, 48 (26.6%) to NS1 and IgG, and 83 (46.1%) were positive to both IgM and IgG and 48(26%) were positive for NS1, IgM and IgG. \n \n A randomly selected subset of 50 serology positive samples were tested by, one step TaqMan real time fourplex RT-PCR assay. A total of 35/50 (70%) samples were found positive by PCR. Positive rates of dengue serotypes were 15/35 (42.8%), 11/35 (31.4%) and 9/35 (25.7%) for DENV-1, DENV-2 and DENV-3 respectively. No DENV-4 serotype was detected.","PeriodicalId":92670,"journal":{"name":"Journal of human virology & retrovirology","volume":"3 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2016-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Epidemiological Profile and Laboratory Characteristics of Dengue Virus Infection during 2011 Outbreak in Rawalpindi, Islamabad Pakistan\",\"authors\":\"M. Suleman, R. Faryal, Mian Muhammad Sufian, Y. Arshad\",\"doi\":\"10.15406/JHVRV.2016.03.00099\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A total of 215 serum samples of clinically suspected dengue patients who presented to the tertiary care hospitals over a period of July-November 2011 were analyzed for the detection of dengue NS1 antigen, dengue IgM and IgG antibodies and 180 (83.7%) samples were found positive by one or more than one testing parameters. Of the 180 samples, 98 (54.4%) were positive to NS1, 168 (93.3%) were positive to IgM and 93 (51.6%) cases were positive to IgG only. Similarly, 93 (51.6%) were positive to both NS1 and IgM, 48 (26.6%) to NS1 and IgG, and 83 (46.1%) were positive to both IgM and IgG and 48(26%) were positive for NS1, IgM and IgG. \\n \\n A randomly selected subset of 50 serology positive samples were tested by, one step TaqMan real time fourplex RT-PCR assay. A total of 35/50 (70%) samples were found positive by PCR. Positive rates of dengue serotypes were 15/35 (42.8%), 11/35 (31.4%) and 9/35 (25.7%) for DENV-1, DENV-2 and DENV-3 respectively. No DENV-4 serotype was detected.\",\"PeriodicalId\":92670,\"journal\":{\"name\":\"Journal of human virology & retrovirology\",\"volume\":\"3 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-07-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of human virology & retrovirology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15406/JHVRV.2016.03.00099\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of human virology & retrovirology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15406/JHVRV.2016.03.00099","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Epidemiological Profile and Laboratory Characteristics of Dengue Virus Infection during 2011 Outbreak in Rawalpindi, Islamabad Pakistan
A total of 215 serum samples of clinically suspected dengue patients who presented to the tertiary care hospitals over a period of July-November 2011 were analyzed for the detection of dengue NS1 antigen, dengue IgM and IgG antibodies and 180 (83.7%) samples were found positive by one or more than one testing parameters. Of the 180 samples, 98 (54.4%) were positive to NS1, 168 (93.3%) were positive to IgM and 93 (51.6%) cases were positive to IgG only. Similarly, 93 (51.6%) were positive to both NS1 and IgM, 48 (26.6%) to NS1 and IgG, and 83 (46.1%) were positive to both IgM and IgG and 48(26%) were positive for NS1, IgM and IgG.
A randomly selected subset of 50 serology positive samples were tested by, one step TaqMan real time fourplex RT-PCR assay. A total of 35/50 (70%) samples were found positive by PCR. Positive rates of dengue serotypes were 15/35 (42.8%), 11/35 (31.4%) and 9/35 (25.7%) for DENV-1, DENV-2 and DENV-3 respectively. No DENV-4 serotype was detected.
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