INVESTIGATION OF THE EFFECT OF ANNEALING TEMPERATURE ON STRUCTURAL, OPTICAL AND ANTIBACTERIAL PROPERTIES OF COPPER OXIDE NANOPARTICLES PREPARED BY FACILE CO-PRECIPITATION ROUTE

Copper oxide (CuO) nanoparticles are important due to their excellent properties and applications such as nano electronics, gas sensors, solar cell and super conductors. Copper oxide have been successfully prepared by using co-precipitation method. CuCl2. 2H2O and NaOH are used in the synthesis of copper oxide nanoparticles as a precursers. Samples annealed at different temperatures such as 400 ºC, 500 ºC, 600 ºC and 700 ºC. The as prepared samples were investigated X-ray diffraction (XRD), scanning electron microscopy (SEM), UV-vis spectroscopy. Their using Antibacterial activity against the two bacterial strain Gram-positive and Gram negative such as (E. coli and B. subtilis) and Hemolysis. Was also investigated XRD analysis showed structure of copper oxide nanoparticles was monoclinic and average crystallite size was found to increase with an increase in annealing temperature in the range of 35 nm to 45 nm. Morphology of SEM images showed that CuO nanostructures were rod like at lower temperature but in higher temperature sphere- like shape was observed. UV-visible spectroscopy results revealed the variation of band gap energy with increasing annealing temperature. Samples prepared at higher temperatures exhibited high antibacterial activity for E. coli and B. subtilis and low toxicity. Low homolytic is safer to use in different applications.