热促进体外紫杉醇给药到猪颈动脉壁

IF 0.8 Q4 ENGINEERING, BIOMEDICAL
R. Homma, Machiko Shinozuka, N. Shimazaki, E. Ogawa, T. Arai
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引用次数: 1

摘要

我们研究了罗丹明B和俄勒冈绿标记紫杉醇(OGLP)在离体猪颈动脉壁(CAW)样品中加热50-70°C 15 s后的递送情况。将分离的CAW样品置于加热过的孔团溶液中,与参比溶液相比,疏水罗丹明B的渗透深度在37℃时显著增加。在70℃加热15 s后,OGLP的穿透深度也有增加的趋势。我们还通过观察加热后CAW样品的内表面结构和疏水性来研究该剂增强递送的机理。加热到70℃以上时,加热后的CAW样品的内表面出现了膨胀的网状结构,介质层的平均疏水性也显著增加。我们假设,在60-70°C下加热15 s,由于CAW内表面的网状结构扩大,同时疏水性增加,因此增强了向CAW样品递送的荧光团。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Heat Enhances Ex Vivo Paclitaxel Delivery to Porcine Carotid Artery Wall
We investigated the delivery of rhodamine B and Oregon Green®-labeled paclitaxel (OGLP) in ex vivo porcine carotid artery wall (CAW) samples after heating the reagents to 50–70°C for 15 s. When the isolated CAW samples were placed in the heated uorophore solutions, the penetration depth of the hydrophobic rhodamine B increased signi cantly compared with reference solution at 37°C. The penetration depth of OGLP also tended to increase upon heating to 70°C for 15 s. We also studied the mechanism of this agent delivery enhancement by observing the inner surface structure and hydrophobicity of the CAW samples after heating. An expanded mesh structure at the inner surface of the heated CAW samples was observed upon heating above 70°C, and the mean hydrophobicity of the media layer also increased signi cantly. We hypothesize that heating at 60–70°C for 15 s enhances the delivery of uorophores to CAW samples as a result of an expanded mesh structure at the inner surface of the CAW, along with a simultaneous increase in hydrophobicity.
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来源期刊
Advanced Biomedical Engineering
Advanced Biomedical Engineering ENGINEERING, BIOMEDICAL-
CiteScore
1.40
自引率
10.00%
发文量
15
审稿时长
15 weeks
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