过氧化磷脂酰胆碱测定方法的改进

Q4 Biochemistry, Genetics and Molecular Biology

Japanese Journal of Clinical Chemistry Pub Date : 1994-06-30 DOI:10.14921/JSCC1971B.23.2_178

K. Seya, N. Ohkohchi, S. Mori

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引用次数: 0

摘要

介绍了一种改进的比色法测定过氧化磷脂酰胆碱的方法。过氧化磷脂酰胆碱在室温下与碘化钾在乙酰氯的存在下反应，在10%的醋酸水溶液中加入淀粉，在550 nm处比色测定释放的碘。在5 ~ 40 nmol之间观察到线性响应。

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An Improved Method for Determination of Phosphatidylcholine Hydroperoxide

An improved colorimetric method for the determination of phosphatidylcholine hydroperoxide is described. Phosphatidylcholine hydroperoxide was allowed to react with potassium iodide in the presence of acetylchloride at room temperature, and the liberated iodine was measured colorimetrically at 550 nm after the addition of starch in 10% aqueous acetic acid. A linear response was observed between 5 and 40 nmol.

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Japanese Journal of Clinical Chemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

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