柱后光化学反应高效液相色谱法测定人尿中萘啶酸

Q4 Biochemistry, Genetics and Molecular Biology

Japanese Journal of Clinical Chemistry Pub Date : 1992-03-31 DOI:10.14921/JSCC1971B.21.1_13

K. Mawatari, F. Iinuma, Mitsuo Watanabe

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引用次数: 2

摘要

建立了采用柱后光化学反应和荧光检测相结合的高效液相色谱法测定人尿中钠啶酸浓度的方法。流动相为0.1 M磷酸二氢钾-四硼酸钠缓冲液(pH 8.6)-甲醇(75:25,v/v)，含20 mM过氧化氢。用紫外光照射柱出水中的化合物以产生荧光。这种荧光在380 nm的激发和440 nm的发射下进行监测。在0.2 ~ 300 ng/支范围内，钠啶酸的校准曲线呈线性关系。尿液的平均回收率接近100%。

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Fluorometric Determination of Nalidixic Acid in Human Urine Using High-Performance Liquid Chromatography with Postcolumn Photochemical Reaction

A high-performance liquid chromatographic method involving a postcolumn photochemical reaction and fluorometric detection has been developed for the determination of nalidixic acid concentration in human urine. The mobile phase consists of 0.1 M potassium dihydrogenphosphate-sodium tetraborate buffer (pH 8.6)-methanol (75 : 25, v/v) containing 20 mM hydrogen peroxide. The compound in the column effluent is irradiated with ultraviolet light to produce fluorescence. This fluorescence is monitored with excitation at 380 nm and emission at 440 nm. The calibration curve for nalidixic acid is linear over the range of 0.2-300 ng/injection. The mean recovery from urine is nearly 100%.

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来源期刊

Japanese Journal of Clinical Chemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

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