ANP和BNP抑制培养的牛肾上腺肾小球细胞分泌醛固酮的作用机制

Q4 Biochemistry, Genetics and Molecular Biology

Japanese Journal of Clinical Chemistry Pub Date : 1993-09-30 DOI:10.14921/JSCC1971B.22.3_156

R. Uzawa, K. Su, Taiichiro Kobayashi, Hiroyuki Watanabe, K. Fukuchi, Y. Takagi, K. Gomi, Chang Gu Kang

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引用次数: 0

摘要

心房利钠肽(ANP) (10-8mol/l)、脑利钠肽(BNP) (10-8mol/l)、蛋白激酶C (PKC)抑制剂H-7 (10-5mol/l)和staurosporin (10-8mol/l)对培养的牛肾上腺肾小球细胞醛固酮分泌的抑制作用比未处理的对照组细胞降低40 ~ 50%。当细胞用10-8mol/l血管紧张素II (All)孵育时，也用ANP、BNP或PKC抑制剂处理，醛固酮的分泌量是未处理细胞的50 - 60%。ANP、BNP和PKC抑制剂抑制PKC的表达，提示PKC在醛固酮分泌中起作用。我们的研究结果表明ANP和BNP可能具有未知的第二信使途径，其中PKC的抑制对前-通路至关重要

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Mechanism of ANP and BNP Action on the Suppression of Aldosterone Secretion by Cultured Bovine Adrenal Glomerulosa Cells

Atrial natriuretic peptide (ANP) (10-8mol/l), brain natriuretic peptide (BNP) (10-8mol/l), the protein kinase C (PKC) inhibitors H-7 (10-5mol/l) and staurosporin (10-8mol/l) suppressed aldosterone secretion in cultured bovine adrenal glomerulosa cells by 40 to 50 % compared with untreated control cells. When cells incubated with 10-8mol/l angiotensin II (All) were also treated with ANP, BNP, or PKC inhibitors, aldosterone secretion was 50 to 60 % of that seen in untreated cells. ANP, BNP and PKC inhibitors suppressing the expression of PKC, suggested that PKC plays a role in aldosterone secretion. Our results suggest that ANP and BNP may have an unknown second messenger pathway in which the suppression of PKC is essential for the ex-

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Japanese Journal of Clinical Chemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

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