咖啡因处理增强了低剂量γ辐照诱导的人白血病细胞染色单体型畸变，但对培养的人正常成纤维细胞没有影响

Q4 Medicine

International Journal of Low Radiation Pub Date : 2011-01-01 DOI:10.1504/IJLR.2011.047176

M. N. Jha, J. Bedford, S. Jha, K. Prasad

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引用次数: 1

摘要

我们研究了咖啡因对低剂量γ辐射诱导的人t细胞白血病细胞(Jurkat t细胞)和两种正常人成纤维细胞系(AG1522和GM2149)染色体损伤的影响。发现低剂量的伽马辐射会以剂量依赖的方式增加正常细胞和癌细胞中染色单体间隙和断裂的水平;然而，癌细胞似乎比正常细胞更敏感。辐射暴露前的咖啡因处理在所有辐射剂量下都显著增加了Jurkat t细胞中染色单体间隙和断裂的水平，但没有增加正常细胞中这些畸变的水平。咖啡因对癌细胞和正常细胞的不同作用机制尚不清楚;然而，g2延迟允许更多的时间来重新连接染色体断裂发生，然后咖啡因消除这种延迟在肿瘤细胞中，而不是在正常细胞中可能解释差异。

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Caffeine treatment enhances low dose γ-irradiation- induced chromatid-type aberrations in human leukaemia cells, but not in human normal fibroblast cells in culture

We investigated the effect of caffeine on low dose γ -radiationinduced chromosomal damage in human T-cell leukaemia cells (Jurkat T-cells) and two normal human fibroblast cell lines (AG1522 and GM2149). Low doses of gamma-radiation were found to increase the levels of chromatid gaps and breaks in a dose-dependent manner in both normal and cancer cells; however, cancer cells appeared to be more sensitive than the normal cells. Caffeine treatment before radiation exposure significantly increased the levels of chromatid gaps and breaks in Jurkat T-cells at all radiation doses, but it did not increase the level of these aberrations in normal cells. The mechanisms of this differential effect of caffeine in cancer cells and normal cells are unknown; however, G2-delay allows more time for rejoining of chromosome breakage to occur, then elimination of this delay by caffeine in tumour cells, not in normal cells might account for difference.

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来源期刊

International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis

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期刊介绍： The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.