丁硫氨酸、亚砜胺、半胱氨酸和二硫代糖醇对低剂量辐射细胞的影响

Q4 Medicine

International Journal of Low Radiation Pub Date : 2014-01-01 DOI:10.1504/IJLR.2014.068257

J. C. Luca, D. López-Larraza

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引用次数: 0

摘要

本研究的目的是确定半胱氨酸、丁硫氨酸亚酰亚胺和二硫代糖醇是否能保护CHO K1细胞免受低剂量x辐射诱导的染色体畸变，以及辐射保护是否与这些硫醇的物理性质有关。实验设计包括四种不同的处理:(a)对照，(b)用5mm的丁硫氨酸亚酰亚胺以及半胱氨酸和二硫代糖醇处理的细胞，(c)用100mgy的x射线处理的细胞，(d)用5mm的每种硫醇和100mgy的x射线处理的细胞。在联合治疗中，所有化合物在照射前30分钟加入，并保留到细胞牺牲(18 h)。三种化合物均未显示出辐射防护作用。这些结果与三种硫醇的电荷和化学结构一致。

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Effect of buthionine sulphoximine, cysteine and dithitreitol in cells exposed to low doses of radiation

The goal of this study is to determine whether cysteimine, buthionine sulphoximide and dithitreitol protect CHO K1 cells against chromosomal aberrations induced by low doses of X–radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of buthionine sulphoximide as well as cysteine and dithitreitol, (c) cells treated with 100 mGy of X–rays and (d) cells treated with 5 mM of each thiol and 100 mGy X–rays. In combined treatment, all chemical compounds were added 30 min before irradiation and remained until cell sacrifice (18 h). None of the three compounds studied showed a radioprotector effect. These results are consistent with the electrical charges and chemical structure of the three thiols.

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来源期刊

International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis

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期刊介绍： The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.