60co辐照下全血H2B、CTP合成酶和PLK3基因的转录表达

Q4 Medicine

International Journal of Low Radiation Pub Date : 2011-06-13 DOI:10.1504/IJLR.2011.040649

V. Jain, B. Das, M. Seshadri

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引用次数: 8

摘要

电离辐射在人体细胞中诱导复杂的分子反应，导致mRNA和蛋白质表达的变化。利用外周血单核细胞(PBMCs)应答电离辐射的功能基因转录状态的数据有限。本研究研究了组蛋白2B、CTP合成酶和PLK3的转录谱。在知情同意的情况下，随机抽取10名健康男性的血液样本。全血辐照分为0.3、0.6、1.0、2.0 Gy 4个剂量组，剂量率0.68 Gy/min。在照射后4小时和立即分离pbmc。提取总RNA，转录成cDNA，进行实时定量PCR。我们的研究结果显示，辐照后4小时H2B和CTP合成酶出现剂量依赖性的显著上调。2.0 Gy时PLK3显著上调(P = 0.007)。总之，这些基因可用于种群监测方案。

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Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60 Co gamma radiation

Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B , CTP synthase and PLK3 were studied. Blood samples were collected from ten random healthy males with informed consent. Whole blood irradiation was done at four different dose groups (0.3, 0.6, 1.0 and 2.0 Gy) at a dose rate of 0.68 Gy/minute. PBMCs were separated immediately as well as 4 hours post-irradiation. Total RNA was isolated, transcribed to cDNA and real-time quantitative PCR was performed. Our results revealed a dose-dependent significant upregulation at H2B and CTP synthase at 4 hours post-irradiation. At PLK3 significant upregulation was observed at 2.0 Gy ( P = 0.007). In conclusion, these genes can be used for population monitoring programme.

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来源期刊

International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis

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期刊介绍： The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.