最小的低聚果糖1-酮糖对双歧杆菌的选择性刺激活性优于低聚果糖合成混合物

Nobuyuki Suzuki, Y. Aiba, H. Takeda, Y. Fukumori, Y. Koga
{"title":"最小的低聚果糖1-酮糖对双歧杆菌的选择性刺激活性优于低聚果糖合成混合物","authors":"Nobuyuki Suzuki, Y. Aiba, H. Takeda, Y. Fukumori, Y. Koga","doi":"10.12938/BIFIDUS.25.109","DOIUrl":null,"url":null,"abstract":"A synthetic mixture of fructo-oligosaccharides (mFOS), consisting largely of nystose (GF 3 ) and a lesser amount of 1-kestose (GF 2 ) has been reported to be selectively utilized by bifidobacteria. In the present study, we tried to identify which fructo-oligosaccharide molecule in mFOS is really involved in the stimulation of bifidobacteria in the gut, using both the gnotobiotic murine model and in vitro culture. 1-Kestose administration to gnotobiotic mice that were associated with human fecal microbiota significantly increased the number of bifidobacteria while mFOS administration was unable to sustain bifidobacteria in these hosts. Moreover a simultaneous decrease in the number of clostridia was found in host mice administered 1-kestose but not in those administered mFOS. The acetate/ propionate ratio in the feces was far higher in host mice administered 1-kestose than in those administered mFOS, suggesting the selective growth activation of bifidobacteria by 1-kestose. The culture study demonstrated that 1-kestose exerts a strong growth-stimulating activity on bifidobacteria but a negligible one on clostridia. On the other hand, nystose was able to stimulate clostridia if the clostridia were exposed to nystose for some time. These results suggest the superiority of 1-kestose to mFOS, which consists largely of nystose, in the selective stimulating activity on bifidobacteria.","PeriodicalId":90114,"journal":{"name":"Bioscience and microflora","volume":"25 1","pages":"109-116"},"PeriodicalIF":0.0000,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.12938/BIFIDUS.25.109","citationCount":"44","resultStr":"{\"title\":\"Superiority of 1-kestose, the Smallest Fructo-oligosaccharide, to a Synthetic Mixture of Fructo-oligosaccharides in the Selective Stimulating Activity on Bifidobacteria\",\"authors\":\"Nobuyuki Suzuki, Y. Aiba, H. Takeda, Y. Fukumori, Y. Koga\",\"doi\":\"10.12938/BIFIDUS.25.109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A synthetic mixture of fructo-oligosaccharides (mFOS), consisting largely of nystose (GF 3 ) and a lesser amount of 1-kestose (GF 2 ) has been reported to be selectively utilized by bifidobacteria. In the present study, we tried to identify which fructo-oligosaccharide molecule in mFOS is really involved in the stimulation of bifidobacteria in the gut, using both the gnotobiotic murine model and in vitro culture. 1-Kestose administration to gnotobiotic mice that were associated with human fecal microbiota significantly increased the number of bifidobacteria while mFOS administration was unable to sustain bifidobacteria in these hosts. Moreover a simultaneous decrease in the number of clostridia was found in host mice administered 1-kestose but not in those administered mFOS. The acetate/ propionate ratio in the feces was far higher in host mice administered 1-kestose than in those administered mFOS, suggesting the selective growth activation of bifidobacteria by 1-kestose. The culture study demonstrated that 1-kestose exerts a strong growth-stimulating activity on bifidobacteria but a negligible one on clostridia. On the other hand, nystose was able to stimulate clostridia if the clostridia were exposed to nystose for some time. These results suggest the superiority of 1-kestose to mFOS, which consists largely of nystose, in the selective stimulating activity on bifidobacteria.\",\"PeriodicalId\":90114,\"journal\":{\"name\":\"Bioscience and microflora\",\"volume\":\"25 1\",\"pages\":\"109-116\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.12938/BIFIDUS.25.109\",\"citationCount\":\"44\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience and microflora\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12938/BIFIDUS.25.109\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience and microflora","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12938/BIFIDUS.25.109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 44

摘要

据报道,双歧杆菌选择性地利用了一种合成的低聚果糖(mFOS)混合物,它主要由乳糖糖(GF - 3)和少量的1-酮糖(GF - 2)组成。在本研究中,我们试图通过小鼠模型和体外培养来确定mFOS中哪个果糖低聚糖分子真正参与了肠道双歧杆菌的刺激。1-Kestose给药与人类粪便微生物群相关的非生物小鼠显著增加了双歧杆菌的数量,而mFOS给药无法维持这些宿主中的双歧杆菌。此外,在给予1-酮糖的宿主小鼠中发现梭菌数量同时减少,而在给予mFOS的宿主小鼠中则没有。1-酮糖组小鼠粪便中乙酸/丙酸比值远高于mFOS组,提示1-酮糖可选择性激活双歧杆菌生长。培养研究表明,1-酮糖对双歧杆菌有较强的促生长活性,而对梭状芽孢杆菌的促生长活性可忽略不计。另一方面,如果梭状芽孢杆菌暴露在霉菌中一段时间,霉菌就能刺激梭状芽孢杆菌。这些结果表明,1-酮糖在选择性刺激双歧杆菌活性方面优于主要由乳糖糖组成的mFOS。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Superiority of 1-kestose, the Smallest Fructo-oligosaccharide, to a Synthetic Mixture of Fructo-oligosaccharides in the Selective Stimulating Activity on Bifidobacteria
A synthetic mixture of fructo-oligosaccharides (mFOS), consisting largely of nystose (GF 3 ) and a lesser amount of 1-kestose (GF 2 ) has been reported to be selectively utilized by bifidobacteria. In the present study, we tried to identify which fructo-oligosaccharide molecule in mFOS is really involved in the stimulation of bifidobacteria in the gut, using both the gnotobiotic murine model and in vitro culture. 1-Kestose administration to gnotobiotic mice that were associated with human fecal microbiota significantly increased the number of bifidobacteria while mFOS administration was unable to sustain bifidobacteria in these hosts. Moreover a simultaneous decrease in the number of clostridia was found in host mice administered 1-kestose but not in those administered mFOS. The acetate/ propionate ratio in the feces was far higher in host mice administered 1-kestose than in those administered mFOS, suggesting the selective growth activation of bifidobacteria by 1-kestose. The culture study demonstrated that 1-kestose exerts a strong growth-stimulating activity on bifidobacteria but a negligible one on clostridia. On the other hand, nystose was able to stimulate clostridia if the clostridia were exposed to nystose for some time. These results suggest the superiority of 1-kestose to mFOS, which consists largely of nystose, in the selective stimulating activity on bifidobacteria.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信