Nobuyuki Suzuki, Y. Aiba, H. Takeda, Y. Fukumori, Y. Koga
{"title":"最小的低聚果糖1-酮糖对双歧杆菌的选择性刺激活性优于低聚果糖合成混合物","authors":"Nobuyuki Suzuki, Y. Aiba, H. Takeda, Y. Fukumori, Y. Koga","doi":"10.12938/BIFIDUS.25.109","DOIUrl":null,"url":null,"abstract":"A synthetic mixture of fructo-oligosaccharides (mFOS), consisting largely of nystose (GF 3 ) and a lesser amount of 1-kestose (GF 2 ) has been reported to be selectively utilized by bifidobacteria. In the present study, we tried to identify which fructo-oligosaccharide molecule in mFOS is really involved in the stimulation of bifidobacteria in the gut, using both the gnotobiotic murine model and in vitro culture. 1-Kestose administration to gnotobiotic mice that were associated with human fecal microbiota significantly increased the number of bifidobacteria while mFOS administration was unable to sustain bifidobacteria in these hosts. Moreover a simultaneous decrease in the number of clostridia was found in host mice administered 1-kestose but not in those administered mFOS. The acetate/ propionate ratio in the feces was far higher in host mice administered 1-kestose than in those administered mFOS, suggesting the selective growth activation of bifidobacteria by 1-kestose. The culture study demonstrated that 1-kestose exerts a strong growth-stimulating activity on bifidobacteria but a negligible one on clostridia. On the other hand, nystose was able to stimulate clostridia if the clostridia were exposed to nystose for some time. These results suggest the superiority of 1-kestose to mFOS, which consists largely of nystose, in the selective stimulating activity on bifidobacteria.","PeriodicalId":90114,"journal":{"name":"Bioscience and microflora","volume":"25 1","pages":"109-116"},"PeriodicalIF":0.0000,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.12938/BIFIDUS.25.109","citationCount":"44","resultStr":"{\"title\":\"Superiority of 1-kestose, the Smallest Fructo-oligosaccharide, to a Synthetic Mixture of Fructo-oligosaccharides in the Selective Stimulating Activity on Bifidobacteria\",\"authors\":\"Nobuyuki Suzuki, Y. Aiba, H. Takeda, Y. Fukumori, Y. Koga\",\"doi\":\"10.12938/BIFIDUS.25.109\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A synthetic mixture of fructo-oligosaccharides (mFOS), consisting largely of nystose (GF 3 ) and a lesser amount of 1-kestose (GF 2 ) has been reported to be selectively utilized by bifidobacteria. In the present study, we tried to identify which fructo-oligosaccharide molecule in mFOS is really involved in the stimulation of bifidobacteria in the gut, using both the gnotobiotic murine model and in vitro culture. 1-Kestose administration to gnotobiotic mice that were associated with human fecal microbiota significantly increased the number of bifidobacteria while mFOS administration was unable to sustain bifidobacteria in these hosts. Moreover a simultaneous decrease in the number of clostridia was found in host mice administered 1-kestose but not in those administered mFOS. The acetate/ propionate ratio in the feces was far higher in host mice administered 1-kestose than in those administered mFOS, suggesting the selective growth activation of bifidobacteria by 1-kestose. The culture study demonstrated that 1-kestose exerts a strong growth-stimulating activity on bifidobacteria but a negligible one on clostridia. On the other hand, nystose was able to stimulate clostridia if the clostridia were exposed to nystose for some time. These results suggest the superiority of 1-kestose to mFOS, which consists largely of nystose, in the selective stimulating activity on bifidobacteria.\",\"PeriodicalId\":90114,\"journal\":{\"name\":\"Bioscience and microflora\",\"volume\":\"25 1\",\"pages\":\"109-116\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.12938/BIFIDUS.25.109\",\"citationCount\":\"44\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience and microflora\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12938/BIFIDUS.25.109\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience and microflora","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12938/BIFIDUS.25.109","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Superiority of 1-kestose, the Smallest Fructo-oligosaccharide, to a Synthetic Mixture of Fructo-oligosaccharides in the Selective Stimulating Activity on Bifidobacteria
A synthetic mixture of fructo-oligosaccharides (mFOS), consisting largely of nystose (GF 3 ) and a lesser amount of 1-kestose (GF 2 ) has been reported to be selectively utilized by bifidobacteria. In the present study, we tried to identify which fructo-oligosaccharide molecule in mFOS is really involved in the stimulation of bifidobacteria in the gut, using both the gnotobiotic murine model and in vitro culture. 1-Kestose administration to gnotobiotic mice that were associated with human fecal microbiota significantly increased the number of bifidobacteria while mFOS administration was unable to sustain bifidobacteria in these hosts. Moreover a simultaneous decrease in the number of clostridia was found in host mice administered 1-kestose but not in those administered mFOS. The acetate/ propionate ratio in the feces was far higher in host mice administered 1-kestose than in those administered mFOS, suggesting the selective growth activation of bifidobacteria by 1-kestose. The culture study demonstrated that 1-kestose exerts a strong growth-stimulating activity on bifidobacteria but a negligible one on clostridia. On the other hand, nystose was able to stimulate clostridia if the clostridia were exposed to nystose for some time. These results suggest the superiority of 1-kestose to mFOS, which consists largely of nystose, in the selective stimulating activity on bifidobacteria.