{"title":"肠道菌群和饲粮植酸对无菌和常规大鼠肠道植酸酶活性的影响","authors":"S. Shinoda, Tsutomu Yoshida","doi":"10.12938/BIFIDUS.23.31","DOIUrl":null,"url":null,"abstract":"Germfree and conventionalized rats were fed a basal or 2% phytate diet. On mucosal phytase activity, the intestinal microflora has no marked effect. Dietary phytate decreased phytase activity in mucosal homogenates, but this effect was not clear in the course of purification. In the germfree intestinal mucosa, the electrophoretic pattern showed two peaks of phytase activity which were different in divalent metal ion requirements. One of these purified phytases did not have any alkaline phosphatase activity.","PeriodicalId":90114,"journal":{"name":"Bioscience and microflora","volume":"23 1","pages":"31-35"},"PeriodicalIF":0.0000,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effects of Intestinal Microflora and Dietary Phytate on Intestinal Phytase Activity in Germfree and Conventionalized Rats\",\"authors\":\"S. Shinoda, Tsutomu Yoshida\",\"doi\":\"10.12938/BIFIDUS.23.31\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Germfree and conventionalized rats were fed a basal or 2% phytate diet. On mucosal phytase activity, the intestinal microflora has no marked effect. Dietary phytate decreased phytase activity in mucosal homogenates, but this effect was not clear in the course of purification. In the germfree intestinal mucosa, the electrophoretic pattern showed two peaks of phytase activity which were different in divalent metal ion requirements. One of these purified phytases did not have any alkaline phosphatase activity.\",\"PeriodicalId\":90114,\"journal\":{\"name\":\"Bioscience and microflora\",\"volume\":\"23 1\",\"pages\":\"31-35\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2004-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioscience and microflora\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12938/BIFIDUS.23.31\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioscience and microflora","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12938/BIFIDUS.23.31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of Intestinal Microflora and Dietary Phytate on Intestinal Phytase Activity in Germfree and Conventionalized Rats
Germfree and conventionalized rats were fed a basal or 2% phytate diet. On mucosal phytase activity, the intestinal microflora has no marked effect. Dietary phytate decreased phytase activity in mucosal homogenates, but this effect was not clear in the course of purification. In the germfree intestinal mucosa, the electrophoretic pattern showed two peaks of phytase activity which were different in divalent metal ion requirements. One of these purified phytases did not have any alkaline phosphatase activity.
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