哺乳动物细胞热处理培养基的近红外光谱研究

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2016-08-01 DOI:10.1255/jnirs.1222

É. Szabó, László Párta, Dénes Zalai, S. Gergely, A. Salgó

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引用次数: 3

摘要

研究了一种化学定义的中国仓鼠卵巢(CHO)细胞培养培养基粉末对不同温度(30°C、50°C和70°C)和不同暴露时间(1小时、7小时和13小时)的热处理反应。根据实验设计方法进行热处理。利用色散和傅立叶变换(FT)近红外(NIR)光谱仪收集对照和处理后粉末的光谱，比较样品组。采用非监督分类为主成分分析和监督分类作为类类比的软独立建模的多变量数据分析来识别治疗引起的样本变化。根据热处理温度设定值对样品进行分离，利用二阶导数近红外光谱对对照样品进行区分。为了评价不同处理对CHO细胞生长速率的影响，还进行了摇瓶培养实验。活细胞密度结果显示，处理温度和处理时间是影响细胞存活率的显著因素(p = 0.05)。此外，与传统摇瓶培养相比，在近红外和ft -近红外测量和光谱分析的情况下，介质粉末变化检测的灵敏度更高。

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Investigation of Heat-Treated Cultivation Medium for Mammalian Cells with near Infrared Spectroscopy

A chemically defined medium powder for Chinese hamster ovary (CHO) cell cultivation was investigated regarding its response to heat treatments with different exposure times (1 hour, 7 hours and 13 hours) and temperatures (30 °C, 50 °C and 70 °C). The heat treatments were performed according to a design of experiments approach. Spectra of the control and the treated powders were collected to compare the sample groups using a dispersive and a Fourier transform (FT) near infrared (NIR) spectrometer. Multivariate data analysis including unsupervised classification as principal component analysis and supervised classification as soft independent modelling of class analogy was employed to identify the treatment-induced variations in the samples. Samples were separated according to the temperature set points of heat treatments and the control samples were successfully discriminated based on second-derivative NIR spectra. In order to evaluate the impact of the treatments on the growth rate of CHO cells, shake flask cultivation experiments were also performed. The viable cell density results showed that both treatment temperature and treatment duration were significant factors (p = 0.05). Additionally, the sensitivity of change detection for medium powders was higher in the case of NIR and FT-NIR measurement and spectrum analysis compared to traditional shake flask cultivation.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.