{"title":"缺血前吸入地氟醚增加离体兔肺缺血再灌注引起的血管渗漏。","authors":"Yoshiaki Oshima, Seiji Sakamoto, Kazumasa Yamasaki, Shinsuke Mochida, Kazumi Funaki, Naoki Moriyama, Akihiro Otsuki, Ryo Endo, Masato Nakasone, Shunsaku Takahashi, Tomomi Harada, Yukari Minami, Yoshimi Inagaki","doi":"10.1186/s40064-016-3741-9","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Isoflurane and sevoflurane protect lungs with ischemia-reperfusion (IR) injury. We examined the influence of desflurane on IR lung injury using isolated rabbit lungs perfused with a physiological salt solution.</p><p><strong>Methods: </strong>The isolated lungs were divided into three groups: IR, desflurane-treated ischemia-reperfusion (DES-IR), and ventilation/perfusion-continued control (Cont) groups (n = 6 per group). In the DES-IR group, inhalation of desflurane at 1 minimum alveolar concentration (MAC) was conducted in a stable 30-min phase. In the IR and DES-IR groups, ventilation/perfusion was stopped for 75 min after the stable phase. Subsequently, they were resumed. Each lung was placed on a balance, and weighed. Weight changes were measured serially throughout this experiment. The coefficient of filtration (K<sub>fc</sub>) was determined immediately before ischemia and 60 min after reperfusion. Furthermore, bronchoalveolar lavage fluid (BALF) was collected from the right bronchus at the completion of the experiment. After the completion of the experiment, the left lung was dried, and the lung wet-to-dry weight ratio (W/D) was calculated.</p><p><strong>Results: </strong>The K<sub>fc</sub> values at 60 min after perfusion were 0.40 ± 0.13 ml/min/mmHg/100 g in the DES-IR group, 0.26 ± 0.07 ml/min/mmHg/100 g in the IR group, and 0.22 ± 0.08 (mean ± SD) ml/mmHg/100 g in the Cont group. In the DES-IR group, the K<sub>fc</sub> at 60 min after the start of reperfusion was significantly higher than in the other groups. In the DES-IR group, W/D was significantly higher than in the Cont group. In the DES-IR group, the BALF concentrations of nitric oxide metabolites were significantly higher than in the other groups. In the DES-IR group, the total amount of vascular endothelial growth factor in BALF was significantly higher than in the Cont group.</p><p><strong>Conclusions: </strong>The pre-inhalation of desflurane at 1 MAC exacerbates pulmonary IR injury in isolated/perfused rabbit lungs.</p>","PeriodicalId":21923,"journal":{"name":"SpringerPlus","volume":"5 1","pages":"2031"},"PeriodicalIF":0.0000,"publicationDate":"2016-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5128009/pdf/","citationCount":"0","resultStr":"{\"title\":\"Desflurane inhalation before ischemia increases ischemia-reperfusion-induced vascular leakage in isolated rabbit lungs.\",\"authors\":\"Yoshiaki Oshima, Seiji Sakamoto, Kazumasa Yamasaki, Shinsuke Mochida, Kazumi Funaki, Naoki Moriyama, Akihiro Otsuki, Ryo Endo, Masato Nakasone, Shunsaku Takahashi, Tomomi Harada, Yukari Minami, Yoshimi Inagaki\",\"doi\":\"10.1186/s40064-016-3741-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Isoflurane and sevoflurane protect lungs with ischemia-reperfusion (IR) injury. We examined the influence of desflurane on IR lung injury using isolated rabbit lungs perfused with a physiological salt solution.</p><p><strong>Methods: </strong>The isolated lungs were divided into three groups: IR, desflurane-treated ischemia-reperfusion (DES-IR), and ventilation/perfusion-continued control (Cont) groups (n = 6 per group). In the DES-IR group, inhalation of desflurane at 1 minimum alveolar concentration (MAC) was conducted in a stable 30-min phase. In the IR and DES-IR groups, ventilation/perfusion was stopped for 75 min after the stable phase. Subsequently, they were resumed. Each lung was placed on a balance, and weighed. Weight changes were measured serially throughout this experiment. The coefficient of filtration (K<sub>fc</sub>) was determined immediately before ischemia and 60 min after reperfusion. Furthermore, bronchoalveolar lavage fluid (BALF) was collected from the right bronchus at the completion of the experiment. After the completion of the experiment, the left lung was dried, and the lung wet-to-dry weight ratio (W/D) was calculated.</p><p><strong>Results: </strong>The K<sub>fc</sub> values at 60 min after perfusion were 0.40 ± 0.13 ml/min/mmHg/100 g in the DES-IR group, 0.26 ± 0.07 ml/min/mmHg/100 g in the IR group, and 0.22 ± 0.08 (mean ± SD) ml/mmHg/100 g in the Cont group. In the DES-IR group, the K<sub>fc</sub> at 60 min after the start of reperfusion was significantly higher than in the other groups. In the DES-IR group, W/D was significantly higher than in the Cont group. In the DES-IR group, the BALF concentrations of nitric oxide metabolites were significantly higher than in the other groups. In the DES-IR group, the total amount of vascular endothelial growth factor in BALF was significantly higher than in the Cont group.</p><p><strong>Conclusions: </strong>The pre-inhalation of desflurane at 1 MAC exacerbates pulmonary IR injury in isolated/perfused rabbit lungs.</p>\",\"PeriodicalId\":21923,\"journal\":{\"name\":\"SpringerPlus\",\"volume\":\"5 1\",\"pages\":\"2031\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-11-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5128009/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"SpringerPlus\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1186/s40064-016-3741-9\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2016/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"Multidisciplinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"SpringerPlus","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s40064-016-3741-9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2016/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Multidisciplinary","Score":null,"Total":0}
引用次数: 0
摘要
背景：异氟醚和七氟醚对缺血再灌注（IR）损伤的肺有保护作用。我们用生理盐溶液灌注离体兔肺，观察地氟醚对IR肺损伤的影响。方法：将离体肺分为IR组、地氟醚处理的缺血再灌注组（DES-IR）组和继续通气/灌注对照组（Cont）组（每组6例）。在DES-IR组中，以最低肺泡浓度（MAC） 1吸入地氟醚，在稳定的30分钟阶段进行。在IR和DES-IR组，稳定期后停止通气/灌注75 min。后来又恢复了。每个肺都被放在天平上称重。在整个实验过程中，连续测量体重变化。在缺血前和再灌注后60分钟分别测定过滤系数（Kfc）。实验结束后取右支气管支气管肺泡灌洗液（BALF）。实验结束后，将左肺干燥，计算肺湿干重比（W/D）。结果：DES-IR组灌流后60 min Kfc值为0.40±0.13 ml/min/mmHg/100 g， IR组为0.26±0.07 ml/min/mmHg/100 g，对照组为0.22±0.08 (mean±SD) ml/mmHg/100 g。DES-IR组再灌注开始后60 min Kfc明显高于其他组。DES-IR组W/D明显高于对照组。DES-IR组一氧化氮代谢物BALF浓度显著高于其他组。DES-IR组BALF中血管内皮生长因子的总量明显高于对照组。结论：1 MAC时预吸入地氟醚可加重离体/灌注兔肺IR损伤。
Desflurane inhalation before ischemia increases ischemia-reperfusion-induced vascular leakage in isolated rabbit lungs.
Background: Isoflurane and sevoflurane protect lungs with ischemia-reperfusion (IR) injury. We examined the influence of desflurane on IR lung injury using isolated rabbit lungs perfused with a physiological salt solution.
Methods: The isolated lungs were divided into three groups: IR, desflurane-treated ischemia-reperfusion (DES-IR), and ventilation/perfusion-continued control (Cont) groups (n = 6 per group). In the DES-IR group, inhalation of desflurane at 1 minimum alveolar concentration (MAC) was conducted in a stable 30-min phase. In the IR and DES-IR groups, ventilation/perfusion was stopped for 75 min after the stable phase. Subsequently, they were resumed. Each lung was placed on a balance, and weighed. Weight changes were measured serially throughout this experiment. The coefficient of filtration (Kfc) was determined immediately before ischemia and 60 min after reperfusion. Furthermore, bronchoalveolar lavage fluid (BALF) was collected from the right bronchus at the completion of the experiment. After the completion of the experiment, the left lung was dried, and the lung wet-to-dry weight ratio (W/D) was calculated.
Results: The Kfc values at 60 min after perfusion were 0.40 ± 0.13 ml/min/mmHg/100 g in the DES-IR group, 0.26 ± 0.07 ml/min/mmHg/100 g in the IR group, and 0.22 ± 0.08 (mean ± SD) ml/mmHg/100 g in the Cont group. In the DES-IR group, the Kfc at 60 min after the start of reperfusion was significantly higher than in the other groups. In the DES-IR group, W/D was significantly higher than in the Cont group. In the DES-IR group, the BALF concentrations of nitric oxide metabolites were significantly higher than in the other groups. In the DES-IR group, the total amount of vascular endothelial growth factor in BALF was significantly higher than in the Cont group.
Conclusions: The pre-inhalation of desflurane at 1 MAC exacerbates pulmonary IR injury in isolated/perfused rabbit lungs.
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