鱼腥草生物活性组分及其主要植物成分对离体大鼠膈肌葡萄糖利用能力的体外研究

Q1 Pharmacology, Toxicology and Pharmaceutics

Advances in Pharmacological Sciences Pub Date : 2016-01-26 DOI:10.1155/2016/2573604

Manish Kumar, S. Prasad, S. Hemalatha

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引用次数: 6

摘要

目标。据报道，鱼腥草全株具有有效的抗高血糖活性。为此，本研究对鱼腥草乙醇提取物生物活性组分在离体大鼠半膈肌中的葡萄糖利用能力进行了研究。方法。采用高效液相色谱法(HPTLC)，对槲皮素中乙醇提取物的水(AQ)、己烷(HEX)、氯仿(CHL)、乙酸乙酯(EA) 4个组分进行了植物化学标准化。此外，通过12组不同的体外实验，对大鼠半隔膜的葡萄糖利用能力进行了评价。在研究中，我们对上述不同的鱼肝草提取物进行了评价，其中以胰岛素为标准，槲皮素为生物学标准。结果。在所有测试组分中，与阴性对照组相比，AQ和EA显著增加了离体大鼠半膈的葡萄糖摄取。此外，AQ组分显著提高了葡萄糖的摄取(p < 0.05)，并且比胰岛素更有效。结论。在AQ和EA组分存在的情况下，横膈对葡萄糖摄取的增加可能归因于槲皮素的存在，槲皮素在这两个组分中的含量分别为7.1和3.2% w/w。

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In Vitro Study on Glucose Utilization Capacity of Bioactive Fractions of Houttuynia cordata in Isolated Rat Hemidiaphragm and Its Major Phytoconstituent

Objective. The whole plant of Houttuynia cordata has been reported to have potent antihyperglycemic activity. Therefore, the present study was undertaken to investigate the glucose utilization capacity of bioactive fractions of ethanol extract of Houttuynia cordata (HC) in isolated rat hemidiaphragm. Methods. All the fractions, that is, aqueous (AQ), hexane (HEX), chloroform (CHL), and ethyl acetate (EA), obtained from ethanol extract of H. cordata were subjected to phytochemical standardization use in quercetin as a marker with the help of HPTLC. Further, glucose utilization capacity by rat hemidiaphragm was evaluated in 12 different sets of in vitro experiments. In the study, different fractions from H. cordata as mentioned above were evaluated, where insulin was used as standard and quercetin as a biological standard. Results. Among all the tested fractions, AQ and EA significantly increased glucose uptake by isolated rat hemidiaphragm compared to negative control. Moreover, AQ fractions enhanced the uptake of glucose significantly (p < 0.05) and was found to be more effective than insulin. Conclusions. The augmentation in glucose uptake by hemidiaphragm in presence of AQ and EA fractions may be attributed to the presence of quercetin, which was found to be 7.1 and 3.2% w/w, respectively, in both the fractions.

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来源期刊

Advances in Pharmacological Sciences PHARMACOLOGY & PHARMACY-

CiteScore

6.40

自引率

0.00%

发文量

审稿时长

14 weeks

期刊介绍： Advances in Pharmacological and Pharmaceutical Sciences is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of experimental and clinical pharmacology, pharmaceutics, medicinal chemistry and drug delivery. Topics covered by the journal include, but are not limited to: -Biochemical pharmacology, drug mechanism of action, pharmacodynamics, pharmacogenetics, pharmacokinetics, and toxicology. -The design and preparation of new drugs, and their safety and efficacy in humans, including descriptions of drug dosage forms. -All areas of medicinal chemistry, such as drug discovery, design and synthesis. -Basic biology of drug and gene delivery through to application and development of these principles, through therapeutic delivery and targeting. Areas covered include bioavailability, controlled release, microcapsules, novel drug delivery systems, personalized drug delivery, and techniques for passing biological barriers.