Preparation of modified oligonucleotides by nicking enzyme amplification reaction

 Functionalized nucleic acids have found applications in chemical biology, bioanalysis, catalysis and nanotechnology.  Modifications can be introduced into the phosphate backbone, sugar moiety and, most often, onto a nucleobase.  Base-modified DNA can be synthesized chemically or enzymatically.  Chemical synthesis is carried out on solid support and utilizes phosphorimidate building blocks.  Enzymatic synthesis of (base-modified) DNA is based on the incorporation of (functionalized) nucleoside triphosphates by a DNA polymerase.