Overexpression of VEGF Induces Bone Formation in the Model of Transplantation of Cultured Bone Cells

85 Introduction The technique of the transplantation of cultured bone cells was firstly developed by Caplan and Bruder [1]. This technique will be useful and applicable for the patients who lost their large segments of bone by suffering bone tumor or etc. Their procedure is as follows; messenchymal stromal cells (MSCs) are isolated from bone marrow and expanded in number in culture. When sufficient number of cells are available, they are loaded into a porous ceramics scaffold and surgically inserted into the excision defect. For clinical application of this bone regeneration therapy. Yoshikawa and Ohgushi improved culture method of osteoblastic cells by introducing the culture method of Maniatopoulos [2]. Several kinds of porous ceramics scaffolds were examined for this implantation system [3-7]. For clinical usage, large size of the scaffold is necessary, however the bigger the size is necessary, the harder the invasion of blood vessel into the central area of scaffold becomes. Osteoblasts Overexpression of VEGF Induces Bone Formation in the Model of Transplantation of Cultured Bone Cells