{"title":"质子核磁共振法测定结构脂质氧化稳定性","authors":"S.P.J. NAMAL SENANAYAKE, FEREIDOON SHAHIDI","doi":"10.1111/j.1745-4522.2007.00081.x","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n <h3> ABSTRACT</h3>\n \n <p> <i>The oxidative stability of enzymatically modified oils (structured lipids) and their unmodified counterparts were assessed using proton nuclear magnetic resonance (<sup>1</sup>H NMR) spectroscopy. This methodology was used to monitor relative changes in the proton absorption pattern of the fatty acids of oils during storage at 60C. Relative changes of aliphatic to olefinic (</i>R<i><sub>ao</sub>) and aliphatic to diallylmethylene (</i>R<i><sub>ad</sub>) proton ratios during oil oxidation were determined by <sup>1</sup>H NMR spectroscopy. An increase in </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> values was obtained over the entire storage period. The oxidative stability of oils was also evaluated using conjugated dienes (CD) determination, 2-thiobarbituric acid reactive substances (TBARS) and headspace volatile analysis. A highly significant correlation (</i>r =<i> 0.930–0.992; </i>P ≤ <i>0.005) existed between the CD values and changes in </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> during oxidation of all oils. The correlation coefficient between TBARS and changes in </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> values was in the range of 0.779–0.983 (</i>P ≤ <i>0.05). A high correlation (</i>r =<i> 0.948–0.996; </i>P ≤ <i>0.005) was found between hexanal content and </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> of oils. Propanal content was also highly correlated (</i>r =<i> 0.950–0.990; </i>P ≤ <i>0.005) with </i>R<i><sub>ao</sub> and </i>R<sub><i>ad</i></sub>.</p>\n </section>\n \n <section>\n \n <h3> PRACTICAL APPLICATIONS</h3>\n \n <p>Assessment of the extent of lipid oxidation in food is of much interest to producers and scientists alike. Proton nuclear magnetic resonance spectroscopy provides a valuable tool for quantitation of oxidation of food lipids. This procedure was used for evaluating the oxidative state of structured lipids. The procedure is rapid and nondestructive, requires a small amount of material, and may be considered as “green” because it uses a very minimum amount of solvent and is readily applicable to edible oils and oils extracted from food and biological samples.</p>\n </section>\n </div>","PeriodicalId":15881,"journal":{"name":"Journal of Food Lipids","volume":"14 3","pages":"217-231"},"PeriodicalIF":0.0000,"publicationDate":"2007-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4522.2007.00081.x","citationCount":"6","resultStr":"{\"title\":\"MEASURING OXIDATIVE STABILITY OF STRUCTURED LIPIDS BY PROTON NUCLEAR MAGNETIC RESONANCE\",\"authors\":\"S.P.J. NAMAL SENANAYAKE, FEREIDOON SHAHIDI\",\"doi\":\"10.1111/j.1745-4522.2007.00081.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <section>\\n \\n <h3> ABSTRACT</h3>\\n \\n <p> <i>The oxidative stability of enzymatically modified oils (structured lipids) and their unmodified counterparts were assessed using proton nuclear magnetic resonance (<sup>1</sup>H NMR) spectroscopy. This methodology was used to monitor relative changes in the proton absorption pattern of the fatty acids of oils during storage at 60C. Relative changes of aliphatic to olefinic (</i>R<i><sub>ao</sub>) and aliphatic to diallylmethylene (</i>R<i><sub>ad</sub>) proton ratios during oil oxidation were determined by <sup>1</sup>H NMR spectroscopy. An increase in </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> values was obtained over the entire storage period. The oxidative stability of oils was also evaluated using conjugated dienes (CD) determination, 2-thiobarbituric acid reactive substances (TBARS) and headspace volatile analysis. A highly significant correlation (</i>r =<i> 0.930–0.992; </i>P ≤ <i>0.005) existed between the CD values and changes in </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> during oxidation of all oils. The correlation coefficient between TBARS and changes in </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> values was in the range of 0.779–0.983 (</i>P ≤ <i>0.05). A high correlation (</i>r =<i> 0.948–0.996; </i>P ≤ <i>0.005) was found between hexanal content and </i>R<i><sub>ao</sub> and </i>R<i><sub>ad</sub> of oils. Propanal content was also highly correlated (</i>r =<i> 0.950–0.990; </i>P ≤ <i>0.005) with </i>R<i><sub>ao</sub> and </i>R<sub><i>ad</i></sub>.</p>\\n </section>\\n \\n <section>\\n \\n <h3> PRACTICAL APPLICATIONS</h3>\\n \\n <p>Assessment of the extent of lipid oxidation in food is of much interest to producers and scientists alike. Proton nuclear magnetic resonance spectroscopy provides a valuable tool for quantitation of oxidation of food lipids. This procedure was used for evaluating the oxidative state of structured lipids. The procedure is rapid and nondestructive, requires a small amount of material, and may be considered as “green” because it uses a very minimum amount of solvent and is readily applicable to edible oils and oils extracted from food and biological samples.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15881,\"journal\":{\"name\":\"Journal of Food Lipids\",\"volume\":\"14 3\",\"pages\":\"217-231\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-08-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1745-4522.2007.00081.x\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Food Lipids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4522.2007.00081.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Food Lipids","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4522.2007.00081.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
MEASURING OXIDATIVE STABILITY OF STRUCTURED LIPIDS BY PROTON NUCLEAR MAGNETIC RESONANCE
ABSTRACT
The oxidative stability of enzymatically modified oils (structured lipids) and their unmodified counterparts were assessed using proton nuclear magnetic resonance (1H NMR) spectroscopy. This methodology was used to monitor relative changes in the proton absorption pattern of the fatty acids of oils during storage at 60C. Relative changes of aliphatic to olefinic (Rao) and aliphatic to diallylmethylene (Rad) proton ratios during oil oxidation were determined by 1H NMR spectroscopy. An increase in Rao and Rad values was obtained over the entire storage period. The oxidative stability of oils was also evaluated using conjugated dienes (CD) determination, 2-thiobarbituric acid reactive substances (TBARS) and headspace volatile analysis. A highly significant correlation (r = 0.930–0.992; P ≤ 0.005) existed between the CD values and changes in Rao and Rad during oxidation of all oils. The correlation coefficient between TBARS and changes in Rao and Rad values was in the range of 0.779–0.983 (P ≤ 0.05). A high correlation (r = 0.948–0.996; P ≤ 0.005) was found between hexanal content and Rao and Rad of oils. Propanal content was also highly correlated (r = 0.950–0.990; P ≤ 0.005) with Rao and Rad.
PRACTICAL APPLICATIONS
Assessment of the extent of lipid oxidation in food is of much interest to producers and scientists alike. Proton nuclear magnetic resonance spectroscopy provides a valuable tool for quantitation of oxidation of food lipids. This procedure was used for evaluating the oxidative state of structured lipids. The procedure is rapid and nondestructive, requires a small amount of material, and may be considered as “green” because it uses a very minimum amount of solvent and is readily applicable to edible oils and oils extracted from food and biological samples.