用微珠定量评价牙本质唾液蛋白(DSP) -一种潜在的牙根吸收的早期标志物。

L. Lombardo, F. Carinci, Mauro Martini, D. Gemmati, M. Nardone, G. Siciliani
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引用次数: 23

摘要

目的比较分析龈沟液(GCF)中牙本质唾液蛋白(DSP)的两种不同方法:传统的酶联免疫吸附法(eLISA)和在酶联免疫吸附分析前涂覆DSP特异性抗体的磁微珠法。材料与方法采用纸条插入上切牙近中沟和远中沟进行正畸治疗12周后,采集6例患者的sgcf,并对两种方法进行分析。结果采用Mann-Whitney非参数检验对结果进行统计分析,结果表明微球法比传统eLISA法具有更高的可靠性和更小的可变性。此外,该方法首次实现了以ng/μl为单位对样品中DSP进行定量分析。结论所建立的微球/eLISA法是一种可靠的定量GCF中DSP的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantitive evaluation of dentin sialoprotein (DSP) using microbeads - a potential early marker of root resorption.
PURPOSE This study had the aim of comparing two different methods of analysing dentin sialoprotein (DSP) in the gingival crevicular fluid (GCF): the conventional eLISA approach and a new method involving the use of magnetic micro-beads coated with an antibody specific for DSP prior to eLISA analysis. MATERIALS AND METHODS GCF was taken from six patients following twelve weeks of orthodontic treatment using paper strips inserted into the mesial and distal sulci of the upper incisors, and analysed using both methods. RESULTS Statistical analysis of the results using the Mann-Whitney non-parametric test showed that the micro-bead approach conferred more reliability and less variability on the conventional eLISA approach. Furthermore, this method, for the first time, enables the quantification of the DSP in the sample in ng/μl. CONCLUSIONS The innovative micro-bead/eLISA approach proposed provides a reliable means of quantifying the DSP in the GCF.
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来源期刊
ORAL and Implantology
ORAL and Implantology Dentistry-Dentistry (all)
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