{"title":"环介导等温扩增检测食源性志贺氏菌的简单快速方法","authors":"SULONG LI, XINBO ZHANG, DEGUO WANG, YANYUN KUANG, YIGANG XU","doi":"10.1111/j.1745-4581.2009.00183.x","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n <h3> ABSTRACT</h3>\n \n <p> <i>A novel method was developed and evaluated for detecting</i> Shigella <i>by a loop-mediated isothermal amplification (LAMP) in this paper. Specific primers were specially designed and selected for recognizing seven distinct sequences of</i> ipaH <i>gene, which was performed only by</i> Shigella. <i>The sensitivity of the LAMP assay was 43 cfu/mL, approximately equal to that of real-time polymerase chain reaction assay. The LAMP assay developed in this study is specific, sensitive and suitable for the detection of foodborne</i> Shigella.</p>\n </section>\n \n <section>\n \n <h3> PRACTICAL APPLICATION</h3>\n \n <p>The LAMP method reported here provided a powerful tool for detection of foodborne <i>Shigella</i> due to its specificity, sensitivity and rapidity.</p>\n </section>\n </div>","PeriodicalId":50067,"journal":{"name":"Journal of Rapid Methods and Automation in Microbiology","volume":"17 4","pages":"465-475"},"PeriodicalIF":0.0000,"publicationDate":"2009-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1745-4581.2009.00183.x","citationCount":"12","resultStr":"{\"title\":\"SIMPLE AND RAPID METHOD FOR DETECTING FOODBORNE SHIGELLA BY A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION\",\"authors\":\"SULONG LI, XINBO ZHANG, DEGUO WANG, YANYUN KUANG, YIGANG XU\",\"doi\":\"10.1111/j.1745-4581.2009.00183.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <section>\\n \\n <h3> ABSTRACT</h3>\\n \\n <p> <i>A novel method was developed and evaluated for detecting</i> Shigella <i>by a loop-mediated isothermal amplification (LAMP) in this paper. Specific primers were specially designed and selected for recognizing seven distinct sequences of</i> ipaH <i>gene, which was performed only by</i> Shigella. <i>The sensitivity of the LAMP assay was 43 cfu/mL, approximately equal to that of real-time polymerase chain reaction assay. The LAMP assay developed in this study is specific, sensitive and suitable for the detection of foodborne</i> Shigella.</p>\\n </section>\\n \\n <section>\\n \\n <h3> PRACTICAL APPLICATION</h3>\\n \\n <p>The LAMP method reported here provided a powerful tool for detection of foodborne <i>Shigella</i> due to its specificity, sensitivity and rapidity.</p>\\n </section>\\n </div>\",\"PeriodicalId\":50067,\"journal\":{\"name\":\"Journal of Rapid Methods and Automation in Microbiology\",\"volume\":\"17 4\",\"pages\":\"465-475\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-12-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1745-4581.2009.00183.x\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Rapid Methods and Automation in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.2009.00183.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Rapid Methods and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1745-4581.2009.00183.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
SIMPLE AND RAPID METHOD FOR DETECTING FOODBORNE SHIGELLA BY A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
ABSTRACT
A novel method was developed and evaluated for detecting Shigella by a loop-mediated isothermal amplification (LAMP) in this paper. Specific primers were specially designed and selected for recognizing seven distinct sequences of ipaH gene, which was performed only by Shigella. The sensitivity of the LAMP assay was 43 cfu/mL, approximately equal to that of real-time polymerase chain reaction assay. The LAMP assay developed in this study is specific, sensitive and suitable for the detection of foodborne Shigella.
PRACTICAL APPLICATION
The LAMP method reported here provided a powerful tool for detection of foodborne Shigella due to its specificity, sensitivity and rapidity.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
