膀胱癌患者膀胱洗涤液中端粒酶催化亚基的临床评价。

Tatsunari Fukui, N. Nonomura, T. Tokizane, Eiichi Sato, Y. Ono, Y. Harada, K. Nishimura, S. Takahara, A. Okuyama
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引用次数: 12

摘要

目的检测端粒酶复合体的催化亚基——人端粒酶逆转录酶(hTERT) mRNA在膀胱冲洗中的表达,作为膀胱癌的肿瘤标志物。材料与方法对63例患者进行膀胱冲洗。我们采用巢式逆转录聚合酶链反应(RT-PCR)方法检测了hTERT mRNA的表达,并通过RT-PCR分析了这些样品中hTERT mRNA和β(2)-微球蛋白(β (2)-MG)的相对表达量。分析hTERT mRNA相对表达量与临床特征的相关性。我们还比较了该试验与尿细胞学的敏感性。结果巢式RT-PCR在对照组中检出3例假阳性(11%)。因此,hTERT mRNA和β (2)-MG的相对表达值与肿瘤大小密切相关,而与肿瘤的多样性和组织学分级无关。当表达截止值固定为0.27%时,该检测的敏感性为74%,特异性为93%。该方法检测膀胱癌的灵敏度高于尿细胞学。结论膀胱冲洗液中hTERT mRNA的相对表达可用于膀胱癌的筛查。相对表达有助于膀胱癌的诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Clinical evaluation of human telomerase catalytic subunit in bladder washings from patients with bladder cancer.
PURPOSE We examined the expression of mRNA of human telomerase reverse transcriptase (hTERT), a catalytic subunit of the telomerase complex, in bladder washings as a tumor marker for the detection of bladder cancer. MATERIALS AND METHODS Bladder washings were obtained from 63 patients. We examined the expression of hTERT mRNA by the nested reverse transcription-polymerase chain reaction (RT-PCR) method and also measured the relative expressions of hTERT mRNA and beta(2)-microglobulin (beta(2)-MG) in these samples by RT-PCR analysis. Correlations between the relative expression of hTERT mRNA and clinical features were analyzed. We also compared the sensitivity of this assay with that of urinary cytology. RESULTS By nested RT-PCR, we detected three false-positive cases (11%) in the control group. Therefore, the relative expression values of hTERT mRNA and beta(2)-MG correlated strongly with tumor size, but not with multiplicity or histologic grade. When the cut-off value of the expression was fixed at 0.27%, the sensitivity and specificity of this assay were 74% and 93%, respectively. This assay was more sensitive than urinary cytology for the detection of bladder cancer. CONCLUSION These results suggest that the relative expression of hTERT mRNA in bladder washings is useful in screening for bladder cancer. Relative expression is of assistance in diagnosing bladder cancer.
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