横向力对光镊垂直压痕测量单元刚度的影响

IF 6.7 3区工程技术 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

International Journal of Optomechatronics Pub Date : 2016-01-02 DOI:10.1080/15599612.2016.1149896

F. Ndoye, M. S. Yousafzai, G. Coceano, S. Bonin, G. Scoles, O. Ka, J. Niemela, D. Cojoc

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引用次数: 10

摘要

摘要:我们研究了光学捕获微珠在细胞膜垂直压痕过程中产生的侧向力，使用后焦平面干涉测量法确定了各个方向的力分量。我们分析了细胞-微珠的相互作用，并表明确实力也有横向分量。利用Hertz模型，我们计算并比较了由总力和垂直力产生的弹性模量，表明差异是重要的，应该考虑总力。为了证实我们的结果，我们分析了两种乳腺癌细胞系的细胞:MDA-MB-231和HBL-100，已知它们具有不同的癌症侵袭性和硬度。

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The influence of lateral forces on the cell stiffness measurement by optical tweezers vertical indentation

ABSTRACT We studied the lateral forces arising during the vertical indentation of the cell membrane by an optically trapped microbead, using back focal plane interferometry to determine force components in all directions. We analyzed the cell-microbead interaction and showed that indeed the force had also lateral components. Using the Hertz model, we calculated and compared the elastic moduli resulting from the total and vertical forces, showing that the differences are important and the total force should be considered. To confirm our results we analyzed cells from two breast cancer cell lines: MDA-MB-231 and HBL-100, known to have different cancer aggressiveness and hence stiffness.

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来源期刊

International Journal of Optomechatronics 工程技术-工程：电子与电气

CiteScore

9.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： International Journal of Optomechatronics publishes the latest results of multidisciplinary research at the crossroads between optics, mechanics, fluidics and electronics. Topics you can submit include, but are not limited to: -Adaptive optics- Optomechanics- Machine vision, tracking and control- Image-based micro-/nano- manipulation- Control engineering for optomechatronics- Optical metrology- Optical sensors and light-based actuators- Optomechatronics for astronomy and space applications- Optical-based inspection and fault diagnosis- Micro-/nano- optomechanical systems (MOEMS)- Optofluidics- Optical assembly and packaging- Optical and vision-based manufacturing, processes, monitoring, and control- Optomechatronics systems in bio- and medical technologies (such as optical coherence tomography (OCT) systems or endoscopes and optical based medical instruments)