非相干光结构照明显微镜的容错算法

IF 6.7 3区工程技术 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

International Journal of Optomechatronics Pub Date : 2015-04-03 DOI:10.1080/15599612.2015.1034904

Kun Wang, Philipp Heidingsfelder, Jun Gao, Liandong Yu, P. Ott

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引用次数: 4

摘要

在这一贡献，我们提出了一个新的算法结构照明显微镜与非相干光。现有的用于确定焦深响应对比度值的算法要求条纹图案照明的高精度相移。该算法对条纹图的不准确相移具有鲁棒性，大大降低了相移的要求，从而降低了显微镜的成本。通过初步实验验证了该算法的可行性，采用低成本的步进电机代替昂贵的压电驱动，实现了弹性导向微动机构对光栅的移动。确定的震源深度响应非常平滑，与理论震源深度响应非常吻合。

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Fault Tolerant Algorithm for Structured Illumination Microscopy with Incoherent Light

In this contribution we present a new algorithm for structured illumination microscopy with incoherent light. Existing algorithms for determining the contrast values of the focal depth response require a high accurate phase shift of the fringe pattern illumination. The presented algorithm, which is robust against inaccurate phase shift of the fringe pattern, reduces significantly the requirements for the phase shift and consequently the costs of the microscope. The new algorithm was tested by a preliminary experiment, whereby the grating was shifted by an elastic guided micro-motion mechanism employing a low-cost stepper motor replacing the conventional expensive piezo drive. The determined focal depth response is very smooth and corresponds very well to the theoretical focal depth response.

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来源期刊

International Journal of Optomechatronics 工程技术-工程：电子与电气

CiteScore

9.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： International Journal of Optomechatronics publishes the latest results of multidisciplinary research at the crossroads between optics, mechanics, fluidics and electronics. Topics you can submit include, but are not limited to: -Adaptive optics- Optomechanics- Machine vision, tracking and control- Image-based micro-/nano- manipulation- Control engineering for optomechatronics- Optical metrology- Optical sensors and light-based actuators- Optomechatronics for astronomy and space applications- Optical-based inspection and fault diagnosis- Micro-/nano- optomechanical systems (MOEMS)- Optofluidics- Optical assembly and packaging- Optical and vision-based manufacturing, processes, monitoring, and control- Optomechatronics systems in bio- and medical technologies (such as optical coherence tomography (OCT) systems or endoscopes and optical based medical instruments)