抵抗素是由大鼠胰岛产生的，在体外调节胰岛素和胰高血糖素的分泌

IF 1.9 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Islets Pub Date : 2016-10-31 DOI:10.1080/19382014.2016.1251538

M. Sassek, E. Pruszyńska‐Oszmałek, P. Kołodziejski, D. Szczepankiewicz, P. Kaczmarek, M. Wieloch, Katarzyna Kurto, L. Nogowski, K. Nowak, M. Strowski, P. Maćkowiak

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引用次数: 8

摘要

抵抗素参与能量稳态、胰岛素抵抗和炎症的调节。抵抗素在胰腺中的潜在表达以及对胰腺内分泌分泌的调节尚未明确，因此，我们从几个层面对其进行了研究。我们通过免疫组织化学和免疫荧光检测了抵抗素在大鼠胰岛中的定位，并通过RT-PCR和Western Blot检测了抵抗素mRNA在这些结构中的潜在存在。此外，我们还研究了抵抗素在胰腺INS-1E β-和ins - g9 α-细胞系以及离体大鼠胰岛中对胰岛素和胰高血糖素分泌的调节作用。我们在大鼠胰岛周围发现了抵抗素的免疫反应性，并在mRNA和蛋白水平上证实了抵抗素的表达。获得的数据表明抵抗素与胰高血糖素在胰腺α-细胞中共定位。此外，我们发现体外抵抗素在正常(6 mM)和高(24 mM)葡萄糖浓度下降低了INS-1E细胞和胰岛的胰岛素分泌，在1 mM葡萄糖浓度下也降低了G9细胞和胰岛的胰高血糖素分泌，而在6 mM葡萄糖浓度下观察到胰高血糖素分泌的刺激。我们的研究结果表明抵抗素可以调节克隆β或α细胞以及胰岛的胰岛素和胰高血糖素的分泌。

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Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion

ABSTRACT Resistin participates in the regulation of energy homeostasis, insulin resistance, and inflammation. The potential expression in pancreas, and modulation of the endocrine pancreas secretion by resistin is not well characterized, therefore, we examined it on several levels. We examined the localization of resistin in rat pancreatic islets by immunohistochemistry and immunofluorescence, and the potential presence of resistin mRNA by RT-PCR and protein by Western Blot in these structures. In addition, we studied the regulation of insulin and glucagon secretion by resistin in pancreatic INS-1E β- and InR-G9 α-cell lines as well as isolated rat pancreatic islets. We identified resistin immunoreactivity in the periphery of rat pancreatic islets and confirmed the expression of resistin at mRNA and protein level. Obtained data indicated that resistin is co-localized with glucagon in pancreatic α-cells. In addition, we found that in vitro resistin decreased insulin secretion from INS-1E cells and pancreatic islets at normal (6 mM) and high (24 mM) glucose concentrations, and also decreased glucagon secretion from G9 cells and pancreatic islets at 1 mM, whereas a stimulation of glucagon secretion was observed at 6 mM glucose. Our results suggest that resistin can modulate the secretion of insulin and glucagon from clonal β or α cells, and from pancreatic islets.

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来源期刊

Islets ENDOCRINOLOGY & METABOLISM-

CiteScore

3.30

自引率

4.50%

发文量

审稿时长

>12 weeks

期刊介绍： Islets is the first international, peer-reviewed research journal dedicated to islet biology. Islets publishes high-quality clinical and experimental research into the physiology and pathology of the islets of Langerhans. In addition to original research manuscripts, Islets is the leading source for cutting-edge Perspectives, Reviews and Commentaries. Our goal is to foster communication and a rapid exchange of information through timely publication of important results using print as well as electronic formats.