具有可切换性能的聚多巴胺锚定聚(2-甲基-2-恶唑啉)/聚(4-乙烯基吡啶)混合刷吸附胃蛋白酶

IF 1.2 4区 化学 Q4 PHYSICS, ATOMIC, MOLECULAR & CHEMICAL
Kangkang He, Fei Hu, Chaoshi Chen, M. Atif, Yanmei Wang
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引用次数: 0

摘要

以聚(2-甲基-2-恶唑啉)/聚(4-乙烯基吡啶)(PMOXA/P4VP)为基材,在聚(多巴胺)(PDA)修饰的底物上同时接枝胺端PMOXA和硫端P4VP,制备了基于PMOXA/P4VP的混合聚合物刷涂。通过x射线光电子能谱、椭偏仪、zeta电位测量和静水接触角测试对涂层进行了表征。结果表明,通过调整PMOXA与P4VP的进料比来控制涂层组分是可行的。此外,混合刷改性表面的zeta电位和水接触角可以通过改变环境pH值和表面成分来调节。最后,采用异硫氰酸荧光素标记胃蛋白酶实验和表面等离子体共振来研究PMOXA/P4VP混合刷对胃蛋白酶的响应性吸附/解吸。结果表明,通过调整PMOXA或P4VP的分数,在pH=3时,PMOXA/P4VP混合刷涂表面可以吸附大量的胃蛋白酶,在pH=7时,解吸效率达到92%以上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Polydopamine anchored poly(2-methyl-2-oxazoline)/poly(4-vinyl pyridine) mixed brushes with switchable properties for pepsin adsorption
Mixed polymer brushes coating based on poly (2-methyl-2-oxazoline)/ poly(4-vinyl pyridine) (PMOXA/P4VP) was prepared by simultaneously grafting amine-terminated PMOXA and thiolterminated P4VP onto poly(dopamine) (PDA)-modified substrates in this work. The coatings were characterized by X-ray photoelectron spectroscopy, ellipsometry, zeta potential measurements, and the static water contact angle tests. The results indicated that it is feasible to control the components of the coating by adjusting the feed ratio of PMOXA to P4VP. Moreover, the zeta potential and the water contact angle of mixed brushes modified surfaces could be tuned by changing the environmental pH value and surface compositions. Finally, fluorescein isothiocyanate-labelled pepsin assay and surface plasmon resonance were performed to investigate the responsive adsorption/desorption of pepsin by PMOXA/P4VP mixed brushes. The results showed that by adjusting the fraction of PMOXA or P4VP, the PMOXA/P4VP mixed brushes coated surfaces could adsorb a high amount of pepsin at pH=3, and achieve a desorption efficiency of over 92% at pH=7.
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来源期刊
Chinese Journal of Chemical Physics
Chinese Journal of Chemical Physics 物理-物理:原子、分子和化学物理
CiteScore
1.90
自引率
10.00%
发文量
2763
审稿时长
3 months
期刊介绍: Chinese Journal of Chemical Physics (CJCP) aims to bridge atomic and molecular level research in broad scope for disciplines in chemistry, physics, material science and life sciences, including the following: Theoretical Methods, Algorithms, Statistical and Quantum Chemistry Gas Phase Dynamics and Structure: Spectroscopy, Molecular Interactions, Scattering, Photochemistry Condensed Phase Dynamics, Structure, and Thermodynamics: Spectroscopy, Reactions, and Relaxation Processes Surfaces, Interfaces, Single Molecules, Materials and Nanosciences Polymers, Biopolymers, and Complex Systems Other related topics
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