Kangkang He, Fei Hu, Chaoshi Chen, M. Atif, Yanmei Wang
{"title":"具有可切换性能的聚多巴胺锚定聚(2-甲基-2-恶唑啉)/聚(4-乙烯基吡啶)混合刷吸附胃蛋白酶","authors":"Kangkang He, Fei Hu, Chaoshi Chen, M. Atif, Yanmei Wang","doi":"10.1063/1674-0068/cjcp2106103","DOIUrl":null,"url":null,"abstract":"Mixed polymer brushes coating based on poly (2-methyl-2-oxazoline)/ poly(4-vinyl pyridine) (PMOXA/P4VP) was prepared by simultaneously grafting amine-terminated PMOXA and thiolterminated P4VP onto poly(dopamine) (PDA)-modified substrates in this work. The coatings were characterized by X-ray photoelectron spectroscopy, ellipsometry, zeta potential measurements, and the static water contact angle tests. The results indicated that it is feasible to control the components of the coating by adjusting the feed ratio of PMOXA to P4VP. Moreover, the zeta potential and the water contact angle of mixed brushes modified surfaces could be tuned by changing the environmental pH value and surface compositions. Finally, fluorescein isothiocyanate-labelled pepsin assay and surface plasmon resonance were performed to investigate the responsive adsorption/desorption of pepsin by PMOXA/P4VP mixed brushes. The results showed that by adjusting the fraction of PMOXA or P4VP, the PMOXA/P4VP mixed brushes coated surfaces could adsorb a high amount of pepsin at pH=3, and achieve a desorption efficiency of over 92% at pH=7.","PeriodicalId":10036,"journal":{"name":"Chinese Journal of Chemical Physics","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Polydopamine anchored poly(2-methyl-2-oxazoline)/poly(4-vinyl pyridine) mixed brushes with switchable properties for pepsin adsorption\",\"authors\":\"Kangkang He, Fei Hu, Chaoshi Chen, M. Atif, Yanmei Wang\",\"doi\":\"10.1063/1674-0068/cjcp2106103\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mixed polymer brushes coating based on poly (2-methyl-2-oxazoline)/ poly(4-vinyl pyridine) (PMOXA/P4VP) was prepared by simultaneously grafting amine-terminated PMOXA and thiolterminated P4VP onto poly(dopamine) (PDA)-modified substrates in this work. The coatings were characterized by X-ray photoelectron spectroscopy, ellipsometry, zeta potential measurements, and the static water contact angle tests. The results indicated that it is feasible to control the components of the coating by adjusting the feed ratio of PMOXA to P4VP. Moreover, the zeta potential and the water contact angle of mixed brushes modified surfaces could be tuned by changing the environmental pH value and surface compositions. Finally, fluorescein isothiocyanate-labelled pepsin assay and surface plasmon resonance were performed to investigate the responsive adsorption/desorption of pepsin by PMOXA/P4VP mixed brushes. The results showed that by adjusting the fraction of PMOXA or P4VP, the PMOXA/P4VP mixed brushes coated surfaces could adsorb a high amount of pepsin at pH=3, and achieve a desorption efficiency of over 92% at pH=7.\",\"PeriodicalId\":10036,\"journal\":{\"name\":\"Chinese Journal of Chemical Physics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Chemical Physics\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1063/1674-0068/cjcp2106103\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHYSICS, ATOMIC, MOLECULAR & CHEMICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Chemical Physics","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1063/1674-0068/cjcp2106103","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSICS, ATOMIC, MOLECULAR & CHEMICAL","Score":null,"Total":0}
Polydopamine anchored poly(2-methyl-2-oxazoline)/poly(4-vinyl pyridine) mixed brushes with switchable properties for pepsin adsorption
Mixed polymer brushes coating based on poly (2-methyl-2-oxazoline)/ poly(4-vinyl pyridine) (PMOXA/P4VP) was prepared by simultaneously grafting amine-terminated PMOXA and thiolterminated P4VP onto poly(dopamine) (PDA)-modified substrates in this work. The coatings were characterized by X-ray photoelectron spectroscopy, ellipsometry, zeta potential measurements, and the static water contact angle tests. The results indicated that it is feasible to control the components of the coating by adjusting the feed ratio of PMOXA to P4VP. Moreover, the zeta potential and the water contact angle of mixed brushes modified surfaces could be tuned by changing the environmental pH value and surface compositions. Finally, fluorescein isothiocyanate-labelled pepsin assay and surface plasmon resonance were performed to investigate the responsive adsorption/desorption of pepsin by PMOXA/P4VP mixed brushes. The results showed that by adjusting the fraction of PMOXA or P4VP, the PMOXA/P4VP mixed brushes coated surfaces could adsorb a high amount of pepsin at pH=3, and achieve a desorption efficiency of over 92% at pH=7.
期刊介绍:
Chinese Journal of Chemical Physics (CJCP) aims to bridge atomic and molecular level research in broad scope for disciplines in chemistry, physics, material science and life sciences, including the following:
Theoretical Methods, Algorithms, Statistical and Quantum Chemistry
Gas Phase Dynamics and Structure: Spectroscopy, Molecular Interactions, Scattering, Photochemistry
Condensed Phase Dynamics, Structure, and Thermodynamics: Spectroscopy, Reactions, and Relaxation Processes
Surfaces, Interfaces, Single Molecules, Materials and Nanosciences
Polymers, Biopolymers, and Complex Systems
Other related topics