{"title":"小鼠精子对来自卵母细胞微环境的因子改变其运动参数和趋化反应","authors":"L. C. GIOJALAS, R. A. ROASIO*","doi":"10.1046/j.1365-2605.1998.00201.x","DOIUrl":null,"url":null,"abstract":"","PeriodicalId":13890,"journal":{"name":"International journal of andrology","volume":"21 4","pages":"201-206"},"PeriodicalIF":0.0000,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1046/j.1365-2605.1998.00201.x","citationCount":"35","resultStr":"{\"title\":\"Mouse spermatozoa modify their motility parameters and chemotactic response to factors from the oocyte microenvironment\",\"authors\":\"L. C. GIOJALAS, R. A. ROASIO*\",\"doi\":\"10.1046/j.1365-2605.1998.00201.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\",\"PeriodicalId\":13890,\"journal\":{\"name\":\"International journal of andrology\",\"volume\":\"21 4\",\"pages\":\"201-206\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1046/j.1365-2605.1998.00201.x\",\"citationCount\":\"35\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of andrology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2605.1998.00201.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of andrology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2605.1998.00201.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 35
摘要
这项工作的目的是评估在卵母细胞微环境因素的影响下,诱导小鼠精子运动变化所需的时间。为了确定潜伏期的影响,即接触卵母细胞产品前的预孵育期,将精子样本孵育15或90分钟,然后暴露于Dulbecco's Modified Eagle's Medium (DMEM)或超排卵卵母细胞粗提取物(CE)中。测定在Zigmond室中进行,其中一个室填充DMEM或CE,另一个室填充精子悬浮液。使用视频显微镜系统跟踪精子。精子活力分析采用半自动客观方法进行评估,并确定以下参数;(1)动态参数:曲线速度、线速度和线性度;(2)渐进式运动:精子呈圆形或线性运动的百分比;(3)定向运动:精子向DMEM或CE移动的百分比。本研究结果表明,当精子在前孵育15 min后接触CE中的可溶性因子时,其动态参数显著增加,进行性运动发生变化,并诱导精子向CE组分定向运动,而较长时间的预孵育并没有显著改变这些影响。另一方面,在培养基存在的情况下(添加或不添加牛血清白蛋白),精子需要更长的孵育时间,以显著增加其动态参数,并改变其渐进运动性,同时保持随机运动方向。