十三排地鼠光感受器嵌合体的无创成像

IF 1.1 4区医学 Q4 NEUROSCIENCES

Visual Neuroscience Pub Date : 2016-01-26 DOI:10.1017/S0952523815000346

Benjamin S. Sajdak, Y. Sulai, C. Langlo, G. Luna, S. Fisher, D. Merriman, A. Dubra

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引用次数: 22

摘要

地松鼠是研究视觉加工、视网膜回路和视锥细胞感光功能的重要模型。本研究利用共聚焦和非共聚焦分光检测自适应光学扫描眼底镜，在790 nm光下对13列地鼠(Ictidomys tridecemlineatus)的光感受器嵌合体进行了无创纵向成像。光感受器密度、间距和Voronoi分析与人类锥体镶嵌一致。在本研究中，高成像成功率和一致的图像质量加强了地鼠作为一个实用的模型，通过细胞尺度上的纵向成像来帮助药物发现和测试。

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Noninvasive imaging of the thirteen-lined ground squirrel photoreceptor mosaic

Abstract Ground squirrels are an increasingly important model for studying visual processing, retinal circuitry, and cone photoreceptor function. Here, we demonstrate that the photoreceptor mosaic can be longitudinally imaged noninvasively in the 13-lined ground squirrel (Ictidomys tridecemlineatus) using confocal and nonconfocal split-detection adaptive optics scanning ophthalmoscopy using 790 nm light. Photoreceptor density, spacing, and Voronoi analysis are consistent with that of the human cone mosaic. The high imaging success rate and consistent image quality in this study reinforce the ground squirrel as a practical model to aid drug discovery and testing through longitudinal imaging on the cellular scale.

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来源期刊

Visual Neuroscience 医学-神经科学

CiteScore

2.20

自引率

5.30%

发文量

审稿时长

>12 weeks

期刊介绍： Visual Neuroscience is an international journal devoted to the publication of experimental and theoretical research on biological mechanisms of vision. A major goal of publication is to bring together in one journal a broad range of studies that reflect the diversity and originality of all aspects of neuroscience research relating to the visual system. Contributions may address molecular, cellular or systems-level processes in either vertebrate or invertebrate species. The journal publishes work based on a wide range of technical approaches, including molecular genetics, anatomy, physiology, psychophysics and imaging, and utilizing comparative, developmental, theoretical or computational approaches to understand the biology of vision and visuo-motor control. The journal also publishes research seeking to understand disorders of the visual system and strategies for restoring vision. Studies based exclusively on clinical, psychophysiological or behavioral data are welcomed, provided that they address questions concerning neural mechanisms of vision or provide insight into visual dysfunction.