Yuan Qiang , Wei Yun-min , Fu Ming-ming , Qiu You-wen , Wen Hong-tao , Zhang Ming-hui , Liu Ying , Ao Jin-xia
{"title":"抗草甘膦转基因大豆PCR-ELISA检测方法的建立","authors":"Yuan Qiang , Wei Yun-min , Fu Ming-ming , Qiu You-wen , Wen Hong-tao , Zhang Ming-hui , Liu Ying , Ao Jin-xia","doi":"10.1016/S1006-8104(16)30046-0","DOIUrl":null,"url":null,"abstract":"<div><p>A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · L<sup>−1</sup>, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.</p></div>","PeriodicalId":58038,"journal":{"name":"Journal of Northeast Agricultural UniversityEnglish Edition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30046-0","citationCount":"0","resultStr":"{\"title\":\"Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean\",\"authors\":\"Yuan Qiang , Wei Yun-min , Fu Ming-ming , Qiu You-wen , Wen Hong-tao , Zhang Ming-hui , Liu Ying , Ao Jin-xia\",\"doi\":\"10.1016/S1006-8104(16)30046-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · L<sup>−1</sup>, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.</p></div>\",\"PeriodicalId\":58038,\"journal\":{\"name\":\"Journal of Northeast Agricultural UniversityEnglish Edition\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1006-8104(16)30046-0\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Northeast Agricultural UniversityEnglish Edition\",\"FirstCategoryId\":\"91\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1006810416300460\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Northeast Agricultural UniversityEnglish Edition","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1006810416300460","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean
A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 µmol · L−1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples.