{"title":"低铁条件对过氧化氢异丙苯诱导的大肠杆菌细胞DNA损伤修复的影响","authors":"L.M.B.O Asad , D.C Medeiros , I Felzenszwalb , A.C Leitão , N.R Asad","doi":"10.1016/S0921-8777(01)00073-8","DOIUrl":null,"url":null,"abstract":"<div><p>In the present study, we evaluated the sensitivity of different <em>Escherichia coli</em> strains to Cumene hydroperoxide (CHP) treatment under distinct conditions of Fe<sup>2+</sup><span> availability. Our results showed that the pretreatment<span><span> with an iron chelator (dipyridyl) protects all the tested strains against CHP toxic effects, but it was not sufficient to abolish the CHP induced mutagenesis. On the other hand, simultaneous pretreatment with both dipyridyl and neocuproine (copper chelator) leads to a complete protection against CHP </span>mutagenic effects. Our data suggest the participation of copper ion in the CHP mutagenesis induced in </span></span><em>E. coli</em>.</p></div>","PeriodicalId":100935,"journal":{"name":"Mutation Research/DNA Repair","volume":"485 4","pages":"Pages 339-344"},"PeriodicalIF":0.0000,"publicationDate":"2001-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0921-8777(01)00073-8","citationCount":"6","resultStr":"{\"title\":\"Effects of low iron conditions on the repair of DNA lesions induced by Cumene hydroperoxide in Escherichia coli cells\",\"authors\":\"L.M.B.O Asad , D.C Medeiros , I Felzenszwalb , A.C Leitão , N.R Asad\",\"doi\":\"10.1016/S0921-8777(01)00073-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In the present study, we evaluated the sensitivity of different <em>Escherichia coli</em> strains to Cumene hydroperoxide (CHP) treatment under distinct conditions of Fe<sup>2+</sup><span> availability. Our results showed that the pretreatment<span><span> with an iron chelator (dipyridyl) protects all the tested strains against CHP toxic effects, but it was not sufficient to abolish the CHP induced mutagenesis. On the other hand, simultaneous pretreatment with both dipyridyl and neocuproine (copper chelator) leads to a complete protection against CHP </span>mutagenic effects. Our data suggest the participation of copper ion in the CHP mutagenesis induced in </span></span><em>E. coli</em>.</p></div>\",\"PeriodicalId\":100935,\"journal\":{\"name\":\"Mutation Research/DNA Repair\",\"volume\":\"485 4\",\"pages\":\"Pages 339-344\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-05-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0921-8777(01)00073-8\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research/DNA Repair\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0921877701000738\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0921877701000738","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of low iron conditions on the repair of DNA lesions induced by Cumene hydroperoxide in Escherichia coli cells
In the present study, we evaluated the sensitivity of different Escherichia coli strains to Cumene hydroperoxide (CHP) treatment under distinct conditions of Fe2+ availability. Our results showed that the pretreatment with an iron chelator (dipyridyl) protects all the tested strains against CHP toxic effects, but it was not sufficient to abolish the CHP induced mutagenesis. On the other hand, simultaneous pretreatment with both dipyridyl and neocuproine (copper chelator) leads to a complete protection against CHP mutagenic effects. Our data suggest the participation of copper ion in the CHP mutagenesis induced in E. coli.
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