Shirong Yu , Yumin Teng , Noel F. Lowndes , Raymond Waters
{"title":"在Saccharomyces cerevisiae MFA2基因的转录区和非转录区，紫外线诱导的环丁烷嘧啶二聚体的核苷酸切除修复需要RAD9、RAD24、RAD16和RAD26","authors":"Shirong Yu , Yumin Teng , Noel F. Lowndes , Raymond Waters","doi":"10.1016/S0921-8777(01)00061-1","DOIUrl":null,"url":null,"abstract":"<div><p><span>In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the </span><em>MFA</em>2 gene in haploid <span><em>Saccharomyces</em><em> cerevisiae</em></span> (<em>S. cerevisiae</em>) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20<!--> <!-->J/m<sup>2</sup> enhances the removal of CPDs induced by a second UV dose of 100<!--> <!-->J/m<sup>2</sup> in the TS and the NTS of <em>MFA</em>2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in <em>rad9</em>, <em>rad24</em>, <em>rad16</em> and <em>rad26</em> cells, indicating two checkpoint genes <em>RAD</em>9 and <em>RAD</em>24, the global repair gene <em>RAD</em>16 and the transcription coupled repair gene <em>RAD</em>26 are essential for inducible NER.</p></div>","PeriodicalId":100935,"journal":{"name":"Mutation Research/DNA Repair","volume":"485 3","pages":"Pages 229-236"},"PeriodicalIF":0.0000,"publicationDate":"2001-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0921-8777(01)00061-1","citationCount":"19","resultStr":"{\"title\":\"RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene\",\"authors\":\"Shirong Yu , Yumin Teng , Noel F. Lowndes , Raymond Waters\",\"doi\":\"10.1016/S0921-8777(01)00061-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the </span><em>MFA</em>2 gene in haploid <span><em>Saccharomyces</em><em> cerevisiae</em></span> (<em>S. cerevisiae</em>) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20<!--> <!-->J/m<sup>2</sup> enhances the removal of CPDs induced by a second UV dose of 100<!--> <!-->J/m<sup>2</sup> in the TS and the NTS of <em>MFA</em>2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in <em>rad9</em>, <em>rad24</em>, <em>rad16</em> and <em>rad26</em> cells, indicating two checkpoint genes <em>RAD</em>9 and <em>RAD</em>24, the global repair gene <em>RAD</em>16 and the transcription coupled repair gene <em>RAD</em>26 are essential for inducible NER.</p></div>\",\"PeriodicalId\":100935,\"journal\":{\"name\":\"Mutation Research/DNA Repair\",\"volume\":\"485 3\",\"pages\":\"Pages 229-236\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0921-8777(01)00061-1\",\"citationCount\":\"19\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation Research/DNA Repair\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0921877701000611\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0921877701000611","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene
In this study, the effect of a prior UV irradiation on the removal of cyclobutane pyrimidine dimers (CPDs) from the transcribed strand (TS) and non-transcribed strand (NTS) of the MFA2 gene in haploid Saccharomyces cerevisiae (S. cerevisiae) cells was investigated. In NER competent cells, the pre-irradiation with a dose of 20 J/m2 enhances the removal of CPDs induced by a second UV dose of 100 J/m2 in the TS and the NTS of MFA2 gene except for the CPDs in the region +258 to +298 in the NTS, where the enhanced repair was absent. No inducible repair was observed in rad9, rad24, rad16 and rad26 cells, indicating two checkpoint genes RAD9 and RAD24, the global repair gene RAD16 and the transcription coupled repair gene RAD26 are essential for inducible NER.
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