伊朗海洋和养殖对虾中金黄色葡萄球菌流行率的研究,旨在开发预防性疫苗

N. Arfatahery, A. Mirshafiey, T.P. Abedimohtasab, M. Zeinolabedinizamani
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引用次数: 18

摘要

金黄色葡萄球菌是海产品中最重要的病原体。由于食用了被这种细菌及其肠道毒素污染的水产品,人类可能会发生食物中毒。为保持和保存这些产品的质量而进行的程序,从它们被捕捞和运输到商店,直到它们被消费,可能在致病菌和毒素的产生和生长中发挥重要作用。共收集了300个样本,包括(新鲜和冷冻,农场和海洋)。为了检测金黄色葡萄球菌,与伊朗国家标准一致,采用了许多表型和分子测定法筛选金黄色葡萄球菌。该研究于2013年9月至2014年3月进行。采用含蛋黄的Baird Parker琼脂和碲酸盐乳剂进行分离。采用凝固酶、脱氧核糖核酸酶、过氧化氢酶、甘露醇发酵、5%羊血琼脂溶血带、VP试验和革兰氏染色鉴定分离株,共74份(24.6%)样品被金黄色葡萄球菌污染。由于虾类中存在金黄色葡萄球菌,渔业部门有必要执行质量控制标准,对海产品的捕捞、养殖、制备、冷冻和运输进行严格监控,并确保工人的健康。研究结果还表明,有必要研制预防金黄色葡萄球菌引起的疾病和海鲜中毒的疫苗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of the Prevalence of Staphylococcus Aureus in Marine and Farmed Shrimps in Iran Aiming the Future Development of a Prophylactic Vaccine

Staphylococcus aureus is the most important pathogen found in sea foods. Food poisoning in human may happen due to the consumption of aqua products contaminated with this bacteria and its enterotoxin. The procedures carried out to maintain and preserve the quality of these products, from the time they are fished and transported to stores until they are consumed, can play a major role in the generation and growth of pathogenic bacteria and toxins. A total of 300 samples were collected, including (fresh and frozen, farm and marine). Consistent with the Iran National Standards, a number of phenotypical and molecular assays were utilized for screening S. aureus in order to detect Staphylococcus aureus. They study was conducted from September 2013 to March 2014. Baird Parker agar containing egg-yolk and tellurite emulsion were used for isolation. Isolates were identified using the following criteria: production of coagulase, DNase, catalase, mannitol fermentation, hemolytic zone on 5% sheep blood agar, VP test and Gramstaining A total of 74 samples (24.6%), were contaminated with Staphylococcus aureus. Due to the presence of Staphylococcus aureus in shrimps, it is necessary to enforce quality control standards by the fisheries and carefully monitor fishing, farming, preparation, freezing, and transporting marine products, and ensure the health of workers. The results of this study also showed it is necessary to produce and develop a vaccine to prevent the disease and sea-food poisoning caused by Staphylococcus aureus.

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