胶质瘤干细胞样细胞的蛋白质组学景观

Q4 Biochemistry, Genetics and Molecular Biology

EuPA Open Proteomics Pub Date : 2015-09-01 DOI:10.1016/j.euprot.2015.06.008

Cheryl F. Lichti , Norelle C. Wildburger , Alexander S. Shavkunov , Ekaterina Mostovenko , Huiling Liu , Erik P. Sulman , Carol L. Nilsson

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引用次数: 9

摘要

胶质瘤干细胞样细胞(GSCs)被假设在肿瘤中提供了一个可以自我复制的细胞库，并且具有放射和化疗抗性。代表几种胶质瘤亚型的GSC细胞系已经被分离出来，并在转录水平上进行了表征。我们试图使用无标记定量蛋白质组学在蛋白质水平上表征35个GSC系。由此产生的相对折叠变化用于驱动无监督分层聚类，目的是基于蛋白质组学谱对细胞系进行分类。生物信息学分析发现滑膜小提琴、丝氨酸/精氨酸丰富剪接因子2、辛普金和IL-5是胶质瘤进展和/或治疗中感兴趣的分子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The proteomic landscape of glioma stem-like cells

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The proteomic landscape of glioma stem-like cells

Glioma stem-like cells (GSCs) are hypothesized to provide a repository of cells in tumors that can self-replicate and are radio- and chemo-resistant. GSC lines, representing several glioma subtypes, have been isolated and characterized at the transcript level. We sought to characterize 35 GSC lines at the protein level using label-free quantitative proteomics. Resulting relative fold changes were used to drive unsupervised hierarchical clustering for the purpose of classifying the cell lines based on proteomic profiles. Bioinformatics analysis identified synoviolin, serine/arginine-rich splicing factor 2, symplekin, and IL-5 as molecules of interest in progression and/or treatment of glioma.

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来源期刊

EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry

自引率

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审稿时长

103 days