痤疮丙酸杆菌的表面、分泌和细胞内蛋白质组分析

Q4 Biochemistry, Genetics and Molecular Biology

EuPA Open Proteomics Pub Date : 2015-12-01 DOI:10.1016/j.euprot.2015.06.003

Yang Yu , Jackson Champer , Jenny Kim

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引用次数: 15

摘要

痤疮丙酸杆菌，在寻常性痤疮等疾病中起着重要作用。然而，对痤疮假单胞杆菌发病机制的了解是有限的。很少有研究调查其蛋白质组，这对疫苗开发至关重要。在这里，我们全面研究了痤疮P.菌株ATCC 6919的蛋白质组，包括分泌、细胞壁、膜和细胞质部分在三种类型的生长介质中。使用Orbitrap质谱计对531种蛋白质进行了定量分析，并对其定位和功能进行了生物信息学分类。有几种，包括PPA1939，一种高表达的表面和分泌蛋白，被确定为潜在的候选疫苗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Analysis of the surface, secreted, and intracellular proteome of Propionibacterium acnes

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Analysis of the surface, secreted, and intracellular proteome of Propionibacterium acnes

Propionibacterium acnes, plays an important role in acne vulgaris and other diseases. However, understanding of the exact mechanisms of P. acnes pathogenesis is limited. Few studies have investigated its proteome, which is essential for vaccine development. Here, we comprehensively investigate the proteome of P. acnes strain ATCC 6919, including secreted, cell wall, membrane, and cytosolic fractions in three types of growth media. A total of 531 proteins were quantified using an Orbitrap mass spectrometer and bioinformatically categorized for localization and function. Several, including PPA1939, a highly expressed surface and secreted protein, were identified as potential vaccine candidates.

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来源期刊

EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry

自引率

0.00%

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审稿时长

103 days