乳腺细胞图谱:MDA-MB-231细胞系的细胞器分析使用同位素标记和无标记分析的密度梯度分离

Q4 Biochemistry, Genetics and Molecular Biology

EuPA Open Proteomics Pub Date : 2015-09-01 DOI:10.1016/j.euprot.2015.06.002

Marianne Sandin , Linn Antberg , Fredrik Levander, Peter James

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引用次数: 0

摘要

细胞内蛋白质在细胞器之间的易位是调控许多细胞功能的重要过程。然而，细胞器很少能被分离到纯度，所以已经开发了几种方法来分析由密度梯度离心获得的分数。我们提出了在人类乳腺细胞系，MDA-MB-231细胞器之间的蛋白质分布的分析使用两种方法:同位素标记和无标记的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

$A Breast Cell Atlas: Organelle analysis of the MDA-MB-231 cell line by density-gradient fractionation using isotopic marking and label-free analysis$

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A Breast Cell Atlas: Organelle analysis of the MDA-MB-231 cell line by density-gradient fractionation using isotopic marking and label-free analysis

Protein translocation between organelles in the cell is an important process that regulates many cellular functions. However, organelles can rarely be isolated to purity so several methods have been developed to analyse the fractions obtained by density gradient centrifugation. We present an analysis of the distribution of proteins amongst organelles in the human breast cell line, MDA-MB-231 using two approaches: an isotopic labelling and a label-free approach.

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EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry

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审稿时长

103 days