Robert J. Phillips , Kate J. Heesom , Johanna Trinder , Andrés López Bernal
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引用次数: 5
摘要
利用Orbitrap质谱串联质量标签(Tandem Mass Tag)的强大蛋白质组学技术,对15名妇女在分娩前和分娩期间的连续血浆样本中蛋白质的相对水平进行了定量分析。1038个蛋白获得定量数据,其中217个蛋白在≥10对样品中被定量。大多数蛋白不受产程影响,产程中有32个蛋白水平较低,产程中有2个蛋白水平升高,t检验有足够的能力确定14个蛋白的表达有显著差异。酶联免疫吸附试验证实急性期反应组分c反应蛋白和血清淀粉样蛋白a显著增加。蛋白组学技术在不断发展,最新技术可以对最低限度预处理的复杂生物样品进行无凝胶分析,可以同时鉴定和定量数百种蛋白质。TMT标记和Orbitrap质谱技术适用于连续母体血浆样本的分析,以确定分娩开始的潜在标记物。
Human maternal plasma proteomic changes with parturition
The powerful proteomic technique of Tandem Mass Tag labelling with Orbitrap mass spectrometry was applied to the quantification of relative levels of proteins in serial plasma samples from 15 women prior to and during labour. Quantitative data were obtained for 1038 proteins, with 217 quantified in ≥10 sample pairs. Most proteins were unaffected by labour, 32 had lower levels in labour, and two were increased in labour, and the t test had sufficient power to determine a significant difference in expression in 14 proteins. ELISA confirmed the significant increase of acute phase response components C-reactive protein and serum amyloid A.
Significance
Proteomic technology is constantly advancing, and the latest techniques enable gel-free analysis of minimally preprocessed, complex biological samples, enabling simultaneous identification and quantification of many hundreds of proteins. The technique of TMT labelling and Orbitrap mass spectrometry is applicable to the analysis of serial maternal plasma samples in order to identify potential markers of the onset of labour.