Maria Lígia R. Macedo, Lúcia Betânia Da S. Andrade, Rosana A. Moraes, Jose Xavier-Filho
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引用次数: 111
摘要
1. 球蛋白部分准备的餐Callosobruchus maculatus-resistant豇豆(豇豆属wiguiculata)种子被证明有害于这个bruchid纳入人工seeds.2。在人工种子中添加2%的抗病种子蛋白后，黄斑弓形虫的生长也受到抗性种子蛋白的强烈影响。从抗性和易感豇豆品种种子中纯化的豇豆绒毛蛋白具有相同的SDS-PAGE图谱，但在非变性聚丙烯酰胺凝胶电泳中具有不同的迁移率。这些结果和我们之前获得的结果(Silva和Xavier-Filho, 1991;Sales et al.， 1992)强烈表明，来自TVu 2027和其他品种的豇豆种子的抗性与维西林分子的存在有关，维西林分子难以被糙肠中蛋白酶消化。
Vicilin variants and the resistance of cowpea (Vigna unguiculata) seeds to the cowpea weevil (Callosobruchus maculatus)
1. A globulin fraction prepared from the meal of Callosobruchus maculatus-resistant cowpea (Vigna wiguiculata) seeds was shown to be detrimental to this bruchid when incorporated in artificial seeds.
2. The performance of C. maculatus was also shown to be strongly hindered by vicilins from resistant seeds when these storage proteins were incorporated in artificial seeds at the level of 2%.
3. The purified vicilins from seeds of both resistant and susceptible cowpea varieties were shown to have the same SDS-PAGE pattern but different mobilities in non-denaturing polyacrylamide gel electrophoresis.
4. These results and previous ones obtained by us (Silva and Xavier-Filho, 1991; Sales et al., 1992) strongly suggest that the resistance of cowpea seeds from the cultivar TVu 2027 and from others bred from it is associated with the presence of vicilin molecules which are refractory to digestion by bruchid midgut proteinases.
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