Fluorimetric detection of cyclodextrins via complexation with fluorescence probe in microcolumn liquid chromatography

Cyclodextrins (CDs) are visualized by fluorimetric detection based on inclusion complexation with fluorescence probes in microcolumn liquid chromatography. Visualization of CDs is attributed to the fact that the fluorescence intensity of 2-p-toluidinylnaphthalene-6-sulfonate or other fluorescence probes increases when it is incorporated into the cavity of CDs. The present detection method can be applied to the determination of CDs contained in horseradish paste. © 2001 John Wiley & Sons, Inc. J Micro Sep 13: 19–23, 2001