诱导小鼠炎症性肠病的方法

Q2 Pharmacology, Toxicology and Pharmaceutics

Current Protocols in Pharmacology Pub Date : 2016-03-18 DOI:10.1002/0471141755.ph0558s72

Byoungwook Bang, Lenard M. Lichtenberger

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引用次数: 18

摘要

实验诱导炎症性肠病(IBD)的动物模型有助于更多地了解疾病的机制基础，确定治疗干预的新靶点，并测试新的治疗方法。该单元提供了五种广泛使用的实验诱导肠道炎症小鼠模型的详细方案:右旋糖酐硫酸钠(DSS)化学诱导结肠炎，通过2,4,6-三硝基苯磺酸(TNBS)半抗原诱导结肠炎，mdr1a - / -小鼠幽门螺杆菌诱导结肠炎，CD4+ CD45RBhi SCID转移性结肠炎模型和IL-10 - / -结肠炎模型。©2016 by John Wiley &儿子,Inc。

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Methods of Inducing Inflammatory Bowel Disease in Mice

Animal models of experimentally induced inflammatory bowel disease (IBD) are useful for understanding more about the mechanistic basis of the disease, identifying new targets for therapeutic intervention, and testing novel therapeutics. This unit provides detailed protocols for five widely used mouse models of experimentally induced intestinal inflammation: chemical induction of colitis by dextran sodium sulfate (DSS), hapten-induced colitis via 2,4,6-trinitrobenzene sulfonic acid (TNBS), Helicobacter-induced colitis in mdr1a ^−/− mice, the CD4⁺ CD45RB^hi SCID transfer colitis model, and the IL-10^−/− colitis model. © 2016 by John Wiley & Sons, Inc.

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Current Protocols in Pharmacology Pharmacology, Toxicology and Pharmaceutics-Pharmacology

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