一种新的血清钙保护蛋白(MRP8/14)颗粒增强免疫比浊法(sCAL turbo)有助于在常规临床实验室环境中区分系统性幼年特发性关节炎和其他疾病。

IF 2.4 Q1 PEDIATRICS
Dirk Foell, Melanie Saers, Carolin Park, Ninna Brix, Mia Glerup, Christoph Kessel, Helmut Wittkowski, Claas Hinze, Lillemor Berntson, Anders Fasth, Charlotte Myrup, Ellen Nordal, Marite Rygg, Henrik Hasle, Birgitte Klug Albertsen, Troels Herlin, Dirk Holzinger, Christian Niederberger, Bernhard Schlüter
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引用次数: 0

摘要

背景:对有不明原因炎症症状的儿童进行鉴别诊断可能具有挑战性。特别是,将系统性幼年特发性关节炎(SJIA)与其他诊断区分开来是困难的。我们最近验证了骨髓相关蛋白8/14复合物(MRP8/14,也称为S100A8/A9复合物或血清钙卫蛋白)作为支持SJIA诊断的有用生物标志物。随后用商业ELISA对结果进行了确认。然而,分析技术的进一步优化对于确保其在常规实验室环境中大规模使用的可行性至关重要。方法:为了评估儿童SJIA的准确性,分析了在自动化实验室仪器上进行的血清钙卫蛋白颗粒增强免疫比浊法(sCAL-turbo)的性能。来自615名儿童的样本可用于诊断SJIA(n = 99),非系统性JIA(n = 169),感染(n = 51)、其他炎症性疾病(n = 126)和急性淋巴细胞白血病(ALL = 147)。此外,还包括来自23名健康对照的样本。结果:sCAL turbo法与以往验证研究中使用的MRP8/14 ELISA法具有良好的相关性(r = 0.99,p 结论:血清钙卫蛋白分析是支持诊断长期发热或炎症性疾病患儿SJIA的有用工具。在这里,我们表明,在自动化实验室仪器上检测血清钙卫蛋白的免疫浊度法可以在临床实验室环境中实施,以促进其在临床实践中作为诊断常规测试的使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A novel serum calprotectin (MRP8/14) particle-enhanced immuno-turbidimetric assay (sCAL turbo) helps to differentiate systemic juvenile idiopathic arthritis from other diseases in routine clinical laboratory settings.

A novel serum calprotectin (MRP8/14) particle-enhanced immuno-turbidimetric assay (sCAL turbo) helps to differentiate systemic juvenile idiopathic arthritis from other diseases in routine clinical laboratory settings.

A novel serum calprotectin (MRP8/14) particle-enhanced immuno-turbidimetric assay (sCAL turbo) helps to differentiate systemic juvenile idiopathic arthritis from other diseases in routine clinical laboratory settings.

A novel serum calprotectin (MRP8/14) particle-enhanced immuno-turbidimetric assay (sCAL turbo) helps to differentiate systemic juvenile idiopathic arthritis from other diseases in routine clinical laboratory settings.

Background: Differential diagnosis in children with signs of unprovoked inflammation can be challenging. In particular, differentiating systemic juvenile idiopathic arthritis (SJIA) from other diagnoses is difficult. We have recently validated the complex of myeloid-related proteins 8/14 (MRP8/14, also known as S100A8/A9 complex or serum calprotectin) as a helpful biomarker supporting the diagnosis of SJIA. The results were subsequently confirmed with a commercial ELISA. However, further optimization of the analytical technology is important to ensure its feasibility for large-scale use in routine laboratory settings.

Methods: To evaluate the accuracy in identifying children with SJIA, the performance of a particle-enhanced immuno-turbidimetric assay for serum calprotectin (sCAL turbo) on an automated laboratory instrument was analyzed. Samples from 615 children were available with the diagnoses SJIA (n = 99), non-systemic JIA (n = 169), infections (n = 51), other inflammatory diseases (n = 126), and acute lymphoblastic leukemia (ALL, n = 147). In addition, samples from 23 healthy controls were included.

Results: The sCAL turbo assay correlated well with the MRP8/14 ELISA used in previous validation studies (r = 0.99, p < 0.001). It could reliably differentiate SJIA from all other diagnoses with significant accuracy (cutoff at 10,500 ng/ml, sensitivity 84%, specificity 94%, ROC area under curve 0.960, p < 0.001).

Conclusions: Serum calprotectin analyses are a helpful tool supporting the diagnosis of SJIA in children with prolonged fever or inflammatory disease. Here, we show that an immuno-turbidimetric assay for detection of serum calprotectin on an automated laboratory instrument can be implemented in clinical laboratory settings to facilitate its use as a diagnostic routine test in clinical practice.

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