Durre Shahwar , Zeba Khan , Mohammad Yunus Khalil Ansari , Younghoon Park
{"title":"化学诱变剂（咖啡因和EMS）和重金属[（Pb（NO3）2和Cd（NO3","authors":"Durre Shahwar , Zeba Khan , Mohammad Yunus Khalil Ansari , Younghoon Park","doi":"10.1016/j.mrgentox.2023.503668","DOIUrl":null,"url":null,"abstract":"<div><p>Mutagenic effectiveness and efficiency are the utmost vital indices to determine the effective and efficient mutagenic concentrations for the generation of high frequency of desirable mutation in mutation breeding. Nevertheless, there are meager study are available for employing effective and efficient concentration of caffeine, EMS, Pb(NO<sub>3</sub>)<sub>2</sub> and Cd(NO<sub>3</sub>)<sub>2</sub> for the crop improvement. Thus, the current study was performed to assess the mutagenic effectiveness and efficiency of caffeine, EMS and heavy metals [Pb(NO<sub>3</sub>)<sub>2</sub> Cd(NO<sub>3</sub>)<sub>2</sub>] and to determine the genetic variability of M<sub>2</sub> and M<sub>3</sub> lentil mutant lines. The frequency of different chlorophyll and chromophyll mutation was found highest at moderate and higher concentrations of chemical mutagens and heavy metals in M<sub>1</sub> and M<sub>2</sub> generation. The highest effectiveness was in 20 ppm Cd(NO<sub>3</sub>)<sub>2</sub>, followed by 20 ppm Pb(NO<sub>3</sub>)<sub>2</sub>, 0.10% EMS, and 0.10% caffeine. The present investigation also showed lower doses of caffeine, EMS, Pb(NO<sub>3</sub>)<sub>2</sub>, and Cd(NO<sub>3</sub>)<sub>2</sub> were more efficient than higher concentrations, and caffeine was found more efficient followed by EMS, Pb(NO<sub>3</sub>)<sub>2</sub>, and Cd(NO<sub>3</sub>)<sub>2</sub>. Furthermore, a broad spectrum of viable mutations affecting different morphological characters of the plants viz., leaves, plant height, growth habits, flowers, pods, and seeds in M<sub>2</sub> and M<sub>3</sub> generation were recorded. Ten morphological mutants showing acceptable agronomic and horticultural features were identified, as genetic resources for further breeding.</p></div>","PeriodicalId":18799,"journal":{"name":"Mutation research. Genetic toxicology and environmental mutagenesis","volume":"890 ","pages":"Article 503668"},"PeriodicalIF":2.3000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Relative mutagenic effectiveness and efficiency of chemical mutagens (Caffeine and EMS) and heavy metals [(Pb(NO3)2 and Cd(NO3)2)] in developing chlorophyll and morphological mutants in lentil\",\"authors\":\"Durre Shahwar , Zeba Khan , Mohammad Yunus Khalil Ansari , Younghoon Park\",\"doi\":\"10.1016/j.mrgentox.2023.503668\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Mutagenic effectiveness and efficiency are the utmost vital indices to determine the effective and efficient mutagenic concentrations for the generation of high frequency of desirable mutation in mutation breeding. Nevertheless, there are meager study are available for employing effective and efficient concentration of caffeine, EMS, Pb(NO<sub>3</sub>)<sub>2</sub> and Cd(NO<sub>3</sub>)<sub>2</sub> for the crop improvement. Thus, the current study was performed to assess the mutagenic effectiveness and efficiency of caffeine, EMS and heavy metals [Pb(NO<sub>3</sub>)<sub>2</sub> Cd(NO<sub>3</sub>)<sub>2</sub>] and to determine the genetic variability of M<sub>2</sub> and M<sub>3</sub> lentil mutant lines. The frequency of different chlorophyll and chromophyll mutation was found highest at moderate and higher concentrations of chemical mutagens and heavy metals in M<sub>1</sub> and M<sub>2</sub> generation. The highest effectiveness was in 20 ppm Cd(NO<sub>3</sub>)<sub>2</sub>, followed by 20 ppm Pb(NO<sub>3</sub>)<sub>2</sub>, 0.10% EMS, and 0.10% caffeine. The present investigation also showed lower doses of caffeine, EMS, Pb(NO<sub>3</sub>)<sub>2</sub>, and Cd(NO<sub>3</sub>)<sub>2</sub> were more efficient than higher concentrations, and caffeine was found more efficient followed by EMS, Pb(NO<sub>3</sub>)<sub>2</sub>, and Cd(NO<sub>3</sub>)<sub>2</sub>. Furthermore, a broad spectrum of viable mutations affecting different morphological characters of the plants viz., leaves, plant height, growth habits, flowers, pods, and seeds in M<sub>2</sub> and M<sub>3</sub> generation were recorded. Ten morphological mutants showing acceptable agronomic and horticultural features were identified, as genetic resources for further breeding.</p></div>\",\"PeriodicalId\":18799,\"journal\":{\"name\":\"Mutation research. 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Relative mutagenic effectiveness and efficiency of chemical mutagens (Caffeine and EMS) and heavy metals [(Pb(NO3)2 and Cd(NO3)2)] in developing chlorophyll and morphological mutants in lentil
Mutagenic effectiveness and efficiency are the utmost vital indices to determine the effective and efficient mutagenic concentrations for the generation of high frequency of desirable mutation in mutation breeding. Nevertheless, there are meager study are available for employing effective and efficient concentration of caffeine, EMS, Pb(NO3)2 and Cd(NO3)2 for the crop improvement. Thus, the current study was performed to assess the mutagenic effectiveness and efficiency of caffeine, EMS and heavy metals [Pb(NO3)2 Cd(NO3)2] and to determine the genetic variability of M2 and M3 lentil mutant lines. The frequency of different chlorophyll and chromophyll mutation was found highest at moderate and higher concentrations of chemical mutagens and heavy metals in M1 and M2 generation. The highest effectiveness was in 20 ppm Cd(NO3)2, followed by 20 ppm Pb(NO3)2, 0.10% EMS, and 0.10% caffeine. The present investigation also showed lower doses of caffeine, EMS, Pb(NO3)2, and Cd(NO3)2 were more efficient than higher concentrations, and caffeine was found more efficient followed by EMS, Pb(NO3)2, and Cd(NO3)2. Furthermore, a broad spectrum of viable mutations affecting different morphological characters of the plants viz., leaves, plant height, growth habits, flowers, pods, and seeds in M2 and M3 generation were recorded. Ten morphological mutants showing acceptable agronomic and horticultural features were identified, as genetic resources for further breeding.
期刊介绍：
Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas:
New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results).
Alternatives to and refinement of the use of animals in genotoxicity testing.
Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials.
Studies of epigenetic changes in relation to genotoxic effects.
The use of structure-activity relationships in predicting genotoxic effects.
The isolation and chemical characterization of novel environmental mutagens.
The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures.
The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing).
MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.
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