{"title":"用于客观实时评估粘膜健康的粘膜阻抗谱","authors":"Priyanka Arora , Jaspreet Singh , Anuraag Jena , Surinder Kumar , Viren Sardana , Siddhartha Sarkar , Lileswar Kaman , Arunanshu Behera , Divya Dahiya , Ritambhra Nada , Cherring Tandup , H.S. Jatana , Usha Dutta","doi":"10.1016/j.tige.2023.03.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and Aims</h3><p>There is a need for a real-time objective tool to assess the mucosal health of the gastrointestinal (GI) tract during endoscopy<span>. Our aim was to develop, design, and validate a low-cost mucosal impedance (MI) device and determine its role in differentiating diseased mucosa from normal mucosa.</span></p></div><div><h3>Methods</h3><p>A biocompatible catheter was designed and developed after multiple iterations. It was validated with a commercially available catheter and histopathological analysis in a blinded manner. Patients undergoing resection of the GI tract were recruited after consent, and the resected specimens were analyzed ex vivo for MI within 10 minutes of resection. An average of 3 MI readings of the visually diseased segment and adjacent normal segment were analyzed. MI values of diseased and non-diseased mucosa were compared.</p></div><div><h3>Results</h3><p>The in-house catheter was validated with a commercially available impedance measuring device. It showed a high degree of positive correlation (rho = 0.616; <em>P</em><span> < 0.001). Two hundred and thirty-two patients (age 46 ± 15 years; 68% females) (180 inflammatory and 52 malignant pathology) who were undergoing abdominal surgery were enrolled. The median impedance value of diseased segments was significantly lower than that of the adjacent normal segments of gut in 130 paired samples [1832(727)Ω vs 2604(1295)Ω; </span><em>P</em> < 0.001]. The MI value of segments containing malignant tissue (n = 50) and inflamed tissue (n = 80) was significantly lower than the MI value of adjacent normal segments of the GI tract [1880(977)Ω vs 2583(1431)Ω; <em>P</em> < 0.001 and 1787(557)Ω vs 2515(1244)Ω; <em>P</em> < 0.001, respectively]. There was a 24% reduction in visually diseased segments [median reduction 712(661)Ω] from adjacent normal segments. A biocompatible endoscopic catheter (3-mm diameter) has been developed and tested in 3 patients and was found to differentiate diseased from normal mucosa.</p></div><div><h3>Conclusion</h3><p>Impedance spectroscopy is an effective real-time, simple, objective tool to differentiate diseased gut mucosa from healthy mucosa.</p></div>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mucosal Impedance Spectroscopy for Objective Real-time Assessment of Mucosal Health\",\"authors\":\"Priyanka Arora , Jaspreet Singh , Anuraag Jena , Surinder Kumar , Viren Sardana , Siddhartha Sarkar , Lileswar Kaman , Arunanshu Behera , Divya Dahiya , Ritambhra Nada , Cherring Tandup , H.S. Jatana , Usha Dutta\",\"doi\":\"10.1016/j.tige.2023.03.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and Aims</h3><p>There is a need for a real-time objective tool to assess the mucosal health of the gastrointestinal (GI) tract during endoscopy<span>. Our aim was to develop, design, and validate a low-cost mucosal impedance (MI) device and determine its role in differentiating diseased mucosa from normal mucosa.</span></p></div><div><h3>Methods</h3><p>A biocompatible catheter was designed and developed after multiple iterations. It was validated with a commercially available catheter and histopathological analysis in a blinded manner. Patients undergoing resection of the GI tract were recruited after consent, and the resected specimens were analyzed ex vivo for MI within 10 minutes of resection. An average of 3 MI readings of the visually diseased segment and adjacent normal segment were analyzed. MI values of diseased and non-diseased mucosa were compared.</p></div><div><h3>Results</h3><p>The in-house catheter was validated with a commercially available impedance measuring device. It showed a high degree of positive correlation (rho = 0.616; <em>P</em><span> < 0.001). Two hundred and thirty-two patients (age 46 ± 15 years; 68% females) (180 inflammatory and 52 malignant pathology) who were undergoing abdominal surgery were enrolled. The median impedance value of diseased segments was significantly lower than that of the adjacent normal segments of gut in 130 paired samples [1832(727)Ω vs 2604(1295)Ω; </span><em>P</em> < 0.001]. The MI value of segments containing malignant tissue (n = 50) and inflamed tissue (n = 80) was significantly lower than the MI value of adjacent normal segments of the GI tract [1880(977)Ω vs 2583(1431)Ω; <em>P</em> < 0.001 and 1787(557)Ω vs 2515(1244)Ω; <em>P</em> < 0.001, respectively]. There was a 24% reduction in visually diseased segments [median reduction 712(661)Ω] from adjacent normal segments. A biocompatible endoscopic catheter (3-mm diameter) has been developed and tested in 3 patients and was found to differentiate diseased from normal mucosa.</p></div><div><h3>Conclusion</h3><p>Impedance spectroscopy is an effective real-time, simple, objective tool to differentiate diseased gut mucosa from healthy mucosa.</p></div>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590030723000429\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590030723000429","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Mucosal Impedance Spectroscopy for Objective Real-time Assessment of Mucosal Health
Background and Aims
There is a need for a real-time objective tool to assess the mucosal health of the gastrointestinal (GI) tract during endoscopy. Our aim was to develop, design, and validate a low-cost mucosal impedance (MI) device and determine its role in differentiating diseased mucosa from normal mucosa.
Methods
A biocompatible catheter was designed and developed after multiple iterations. It was validated with a commercially available catheter and histopathological analysis in a blinded manner. Patients undergoing resection of the GI tract were recruited after consent, and the resected specimens were analyzed ex vivo for MI within 10 minutes of resection. An average of 3 MI readings of the visually diseased segment and adjacent normal segment were analyzed. MI values of diseased and non-diseased mucosa were compared.
Results
The in-house catheter was validated with a commercially available impedance measuring device. It showed a high degree of positive correlation (rho = 0.616; P < 0.001). Two hundred and thirty-two patients (age 46 ± 15 years; 68% females) (180 inflammatory and 52 malignant pathology) who were undergoing abdominal surgery were enrolled. The median impedance value of diseased segments was significantly lower than that of the adjacent normal segments of gut in 130 paired samples [1832(727)Ω vs 2604(1295)Ω; P < 0.001]. The MI value of segments containing malignant tissue (n = 50) and inflamed tissue (n = 80) was significantly lower than the MI value of adjacent normal segments of the GI tract [1880(977)Ω vs 2583(1431)Ω; P < 0.001 and 1787(557)Ω vs 2515(1244)Ω; P < 0.001, respectively]. There was a 24% reduction in visually diseased segments [median reduction 712(661)Ω] from adjacent normal segments. A biocompatible endoscopic catheter (3-mm diameter) has been developed and tested in 3 patients and was found to differentiate diseased from normal mucosa.
Conclusion
Impedance spectroscopy is an effective real-time, simple, objective tool to differentiate diseased gut mucosa from healthy mucosa.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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