评估主动脉中膜组织学变化与死亡原因的关系。

IF 1.2 4区 医学 Q4 DEVELOPMENTAL BIOLOGY
Doru Adrian Şeicaru, Mircea Liţescu, Florentina Gherghiceanu, Mircea Sebastian Şerbănescu, Valentin Titus Grigorean, Răzvan Mihail Pleşea
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引用次数: 0

摘要

目的:作者开始评估三种主要形态主动脉参数(弹性纤维-FE、胶原纤维-FCOL和平滑肌纤维-FM)与死亡原因之间的相关性。材料和方法:研究组包括25例死于血管性疾病(V_P),37例死于非血管性疾病,28例死于暴力/可疑非病理性死因(V_Dth),后者也是对照组。尸检期间,从选定病例中收集四个主动脉横截面(基底、主动脉弓、胸部和腹部),固定在10%缓冲福尔马林中,首先用校准尺一起拍照。然后,将它们包埋在石蜡中,在4μm处切片,并用苏木精-曙红(HE)和Orcein染色。将获得的组织学载玻片转化为虚拟载玻片。纤维成分的量是使用在MATLAB(MathWorks,美国)中开发的定制软件。使用的统计工具是Pearson相关检验、t检验(假设方差相等的两个样本)和单因素方差分析(ANOVA)检验。结果和讨论:在三组中的每一组中,主动脉中膜的三种原纤维成分的量在所有主动脉区域都有同步变化,但死于血管病理的患者组中的FCOL除外,其仅呈现沿主动脉同步变化的趋势。在死于血管病理的患者中,FE的值最低,FCOL的值最高。在死于任何病理状况(血管性或非血管性)的人中,FCOL的水平总是高于FE。FM的水平总是比其他类型的纤维低至少两倍,无论患者是否死于病理状况。结论:引起死亡的不同病理条件影响主动脉中膜的纤维组成。需要进一步的研究来揭示主动脉壁和血管壁形态的其他变化,这些变化可能与影响整个生物体的不同病理条件有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of the aortic tunica media histological changes in relation with the cause of death.

Aim: The authors set out to evaluate the correlations between three of the main morphological aortic parameters (elastic fibers - FE, collagen fibers - FCOL, and smooth muscle fibers - FM) and the cause of death.

Materials and methods: Study groups included 25 cases died of a vascular disease (V_P), 37 cases died of a non-vascular disease (NV_P) and 28 cases died of a violent/suspect non-pathological cause of death (V_Dth), the latter group representing also the control group. Four aortic cross-sections (base, arch, thoracic, and abdominal regions) were collected during autopsy from the selected cases, fixed in 10% buffered formalin and first of all photographed together with a calibrating ruler. Then, they were embedded in paraffin, sectioned off at 4 μm and stained with Hematoxylin-Eosin (HE) and Orcein. The obtained histological slides were transformed into virtual slides. Fibrillary components amounts were using a custom-made software, developed in MATLAB (MathWorks, USA). Statistical tools used were Pearson's correlation test, t-test (two-sample assuming equal variances) and one-way analysis of variance (ANOVA) test.

Results and discussions: The amounts of the three fibrillary components of the aortic tunica media had a synchronous variation in all aortic regions in each of the three groups, excepting FCOL in the group of patients died from vascular pathology, which presented only a trend of synchronous variation along the aorta. FE had their lowest values and FCOL had their highest values in patients died from vascular pathology. FCOL had always higher levels than FE in people died from any pathological condition, vascular or non-vascular. FM had always at least two times lower level than that of the other types of fibers, regardless of whether the person died due to a pathological condition or not.

Conclusions: The different pathological conditions causing death are influencing the fibrillary composition of aortic tunica media. Further studies are required to reveal other changes in the morphology of aortic wall in particular and vascular wall in general that could be related with different pathological conditions affecting the entire organism.

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来源期刊
CiteScore
1.70
自引率
20.00%
发文量
221
审稿时长
3-8 weeks
期刊介绍: Romanian Journal of Morphology and Embryology (Rom J Morphol Embryol) publishes studies on all aspects of normal morphology and human comparative and experimental pathology. The Journal accepts only researches that utilize modern investigation methods (studies of anatomy, pathology, cytopathology, immunohistochemistry, histochemistry, immunology, morphometry, molecular and cellular biology, electronic microscopy, etc.).
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