高压增加BV2小胶质细胞TNF-α和IL-1β的表达

Q4 Nursing

中华急诊医学杂志 Pub Date : 2019-12-10 DOI:10.3760/CMA.J.ISSN.1671-0282.2019.12.009

杨帆, Yang Fan, 李旭声, Li Xusheng, 李娅, Li Ya, 刘小瑜, L. Xiaoyu, 刘梦婷, Liu Mengting, 丁洪光, Ding Hong-guang, 曾红科, Zeng Hong-ke

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引用次数: 0

摘要

目的探讨高压是否会增加TNF-a和IL-1β的表达。方法用自制装置处理BV2小胶质细胞。BV2小胶质细胞根据不同压力随机分为5组：对照组、20mmHg组、25mmHg组，30mmHg组和35mmHg组。根据干预时间的不同，将BV2小胶质细胞随机分为5组：对照组、6h组、12h组、24h组。α和IL-1β的表达。结果30mmHg组小胶质细胞TNF-α和IL-1β表达水平高于对照组（均P＜0.05）。关键词：压力；小胶质细胞；TNF-α；IL-1β

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High pressure increases the express of TNF-α and IL-1β in BV2 microglia

Objective This study aimed to explore whether high pressure would increase expression of TNF-a and IL-1β. Methods BV2 microglia cells were treated with a self-made device. BV2 microglia cells were randomly divided into five groups according to different pressures: control group, 20 mmHg group, 25 mmHg group, 30 mmHg group, and 35 mmHg group. BV2 microglia cells were randomly divided into five groups according to different intervention time: control group, 6 h group, 12 h group, 24 h group. TNF-α and IL-1β expression were assessed by Western Blotting or double immunofluorescence. Results The 30 mmHg group had the highest expression levels of TNF-α and IL-1β as compared with control group (both P 0.05). Conclusions High pressure may increase the expression levels of TNF-α and IL-1β of microglia. Key words: Pressure; Microglia; TNF-α; IL-1β

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来源期刊

中华急诊医学杂志 Nursing-Emergency Nursing

CiteScore

0.10

自引率

0.00%

发文量

8629

期刊介绍： Chinese Journal of Emergency Medicine is the only national journal which represents the development of emergency medicine in China. The journal is supervised by China Association of Science and Technology, sponsored by Chinese Medical Association, and co-sponsored by Zhejiang University. The journal publishes original research articles dealing with all aspects of clinical practice and research in emergency medicine. The columns include Pre-Hospital Rescue, Emergency Care, Trauma, Resuscitation, Poisoning, Disaster Medicine, Continuing Education, etc. It has a wide coverage in China, and builds up communication with Hong Kong, Macao, Taiwan and international emergency medicine circles.