波兰卢布林省专科医院分离的具有临床意义的肠球菌的表型和基因型耐药性分析

IF 0.4 Q3 MEDICINE, GENERAL & INTERNAL
Agnieszka Bogut, Patrycja Mrozik, Gabriela Czaja, Małgorzata Stawecka-Hamerla
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引用次数: 0

摘要

摘要肠球菌作为医疗保健相关病原体的重要性日益增加,可能与它们对抗生素的易感性有限有关。在这项研究中,分析了从住院患者培养的35个[n=18个粪便大肠杆菌(Efm);n=17个粪便大肠菌(Efs)]侵袭性分离株的表型和基因型抗性谱。通过靶向16S rDNA和编码Efs和Efm特异性连接酶的ddl基因的多重PCR验证表型鉴定。采用纸片扩散法和E试验测定抗菌药物的敏感性。通过扩增ant(6)-Ia、aac(6’)-Ie-aph(2’’)-Ia以及vanA和vanB基因,分别验证了高水平链霉素耐药性(HLSR)、高水平庆大霉素耐药性(HLGR)和糖肽耐药性。70%以上的分离株是从重症监护室和内科病房的患者身上培养出来的。血液是主要的隔离部位(77%)。所有Efm分离株均对氨苄青霉素、亚胺培南和诺氟沙星具有耐药性;17个分离株表现出高水平氨基糖苷类耐药性(HLAR),包括27.7%的HLSR、38.8%的HLGR和27.7%的两种表型。HLAR在Efs中也很常见(HLSR>70%,HLGR>50%),其次是诺氟沙星(64.7%)和氨苄青霉素(11.7%)耐药性。在90%以上的HLSR和HLGR分离株中分别检测到ant(6)-Ia和aac(6’)-Ie-aph(2’’)-Ia基因。在4株(22.2%)Efm分离株中检测到糖肽抗性,并由vanA基因介导。19株(54.3%)具有多药耐药性,其中Efm 17株(89.5%)。所有分离株均对利奈唑胺敏感。该研究有助于分析波兰医院的肠球菌耐药性。监测肠球菌流行率和抗微生物耐药性对于控制和预防感染至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Analysis of the phenotypic and genotypic antimicrobial resistance profiles of clinically significant enterococci isolated in the Provincial Specialist Hospital in Lublin, Poland
Abstract The increasing significance of enterococci as healthcare-associated pathogens can be linked to their limited susceptibility to antibiotics. In this study, phenotypic and genotypic resistance profiles of 35 [n=18 E. faecium (Efm); n=17 E. faecalis (Efs)] invasive isolates cultured from hospitalized patients were analysed. Phenotypic identification was verified by the multiplex PCR targeting the 16S rDNA and the ddl genes encoding for the Efs and Efm – specific ligases. Antimicrobial susceptibility was determined using the disc diffusion method and E-tests. The high-level streptomycin resistance (HLSR), high-level gentamicin resistance (HLGR) and glycopeptide resistance was verified by amplification of the ant(6)-Ia, aac(6’)-Ie-aph(2’’)-Ia, as well as vanA and vanB genes, respectively. More than 70% of all isolates were cultured from patients in the Intensive Care and Internal Medicine Units. Blood was the predominant (77%) site of isolation. All Efm isolates were resistant to ampicillin, imipenem, and norfloxacin; 17 isolates demonstrated high-level aminoglycoside resistance (HLAR), including 27.7% with HLSR, 38.8% with HLGR and 27.7% with both phenotypes. HLAR was also common in Efs (HLSR>70%, HLGR>50%), followed by norfloxacin (64.7%) and ampicillin (11.7%) resistance. The ant(6)-Ia and aac(6’)-Ie-aph(2’’)-Ia genes were detected in >90% of the HLSR and HLGR isolates, respectively. Glycopeptide resistance was detected in 4 (22.2%) Efm isolates and mediated by the vanA gene. 19 (54.3%) isolates were multidrug resistant, including 17 (89.5%) Efm. All isolates were susceptible to linezolid. The study constitutes a contribution to the analysis of enterococcal antimicrobial resistance in Polish hospitals. The monitoring of enterococcal prevalence and antimicrobial resistance is crucial to control and prevent infections.
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来源期刊
Current Issues in Pharmacy and Medical Sciences
Current Issues in Pharmacy and Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
0.80
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0.00%
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28
审稿时长
16 weeks
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