城市污水进水微生物群落及抗生素抗性基因谱对厌氧膜生物反应器出水的影响

IF 2.3 Q2 Environmental Science
Christelle Sawaya, Charbel El Khoury, Lama Ramadan, Reem Deeb, M. Harb
{"title":"城市污水进水微生物群落及抗生素抗性基因谱对厌氧膜生物反应器出水的影响","authors":"Christelle Sawaya, Charbel El Khoury, Lama Ramadan, Reem Deeb, M. Harb","doi":"10.2166/wrd.2022.018","DOIUrl":null,"url":null,"abstract":"\n Municipal wastewater management is an important target area for reducing the spread of antibiotic resistance, especially given the parallel increasing need for water reuse. Anaerobic membrane bioreactors (AnMBRs) have the potential to play a key role in safely expanding non-potable wastewater reuse practices. In the present study, the effect of the commencing treatment of municipal wastewater by an AnMBR was evaluated after an extended startup phase using only synthetic wastewater. Antibiotic resistance genes (ARGs) associated with sulfonamides, tetracyclines, and β-lactams were quantified, and effluent microbial community progression was analyzed. Results indicated that the AnMBR effluent inherently harbored all targeted ARGs prior to the introduction of real wastewater (104–109 copies/100 mL effluent). sul1, sul2, and intI1 genes were notably higher initially than other genes and markedly increased after the system was transitioned to municipal wastewater. Although potentially pathogenic bacteria made up over 20% relative abundance of the influent, AnMBR effluents showed a marginalization of these groups as their microbial communities more closely resembled the tightly bound layer of membrane biofilms. This work highlights the need for emerging treatment systems to be evaluated on a basis that incorporates the differentiation of system-associated ARGs and assesses their environmental transmission potential within the effluent communities.","PeriodicalId":17556,"journal":{"name":"Journal of Water Reuse and Desalination","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2022-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Effects of influent municipal wastewater microbial community and antibiotic resistance gene profiles on the anaerobic membrane bioreactor effluent\",\"authors\":\"Christelle Sawaya, Charbel El Khoury, Lama Ramadan, Reem Deeb, M. Harb\",\"doi\":\"10.2166/wrd.2022.018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n Municipal wastewater management is an important target area for reducing the spread of antibiotic resistance, especially given the parallel increasing need for water reuse. Anaerobic membrane bioreactors (AnMBRs) have the potential to play a key role in safely expanding non-potable wastewater reuse practices. In the present study, the effect of the commencing treatment of municipal wastewater by an AnMBR was evaluated after an extended startup phase using only synthetic wastewater. Antibiotic resistance genes (ARGs) associated with sulfonamides, tetracyclines, and β-lactams were quantified, and effluent microbial community progression was analyzed. Results indicated that the AnMBR effluent inherently harbored all targeted ARGs prior to the introduction of real wastewater (104–109 copies/100 mL effluent). sul1, sul2, and intI1 genes were notably higher initially than other genes and markedly increased after the system was transitioned to municipal wastewater. Although potentially pathogenic bacteria made up over 20% relative abundance of the influent, AnMBR effluents showed a marginalization of these groups as their microbial communities more closely resembled the tightly bound layer of membrane biofilms. This work highlights the need for emerging treatment systems to be evaluated on a basis that incorporates the differentiation of system-associated ARGs and assesses their environmental transmission potential within the effluent communities.\",\"PeriodicalId\":17556,\"journal\":{\"name\":\"Journal of Water Reuse and Desalination\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2022-08-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Water Reuse and Desalination\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2166/wrd.2022.018\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Water Reuse and Desalination","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2166/wrd.2022.018","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 4

摘要

城市污水管理是减少抗生素耐药性传播的一个重要目标领域,特别是考虑到水再利用需求的同时增加。厌氧膜生物反应器(AnMBRs)有可能在安全扩大非饮用水再利用实践中发挥关键作用。在本研究中,在仅使用合成废水的延长启动阶段后,评估了AnMBR开始处理城市废水的效果。对与磺酰胺类、四环素类和β-内酰胺类相关的抗生素耐药性基因(ARGs)进行了定量,并分析了出水微生物群落的进展。结果表明,在引入真正的废水(104–109拷贝/100 mL废水)之前,AnMBR废水固有地含有所有靶向ARG。sul1、sul2和intI1基因最初显著高于其他基因,并在系统转化为城市污水后显著增加。尽管潜在致病菌占进水相对丰度的20%以上,但AnMBR出水显示出这些类群的边缘化,因为它们的微生物群落更像膜生物膜的紧密结合层。这项工作强调了对新兴处理系统进行评估的必要性,该系统应结合系统相关ARG的差异,并评估其在污水社区内的环境传播潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of influent municipal wastewater microbial community and antibiotic resistance gene profiles on the anaerobic membrane bioreactor effluent
Municipal wastewater management is an important target area for reducing the spread of antibiotic resistance, especially given the parallel increasing need for water reuse. Anaerobic membrane bioreactors (AnMBRs) have the potential to play a key role in safely expanding non-potable wastewater reuse practices. In the present study, the effect of the commencing treatment of municipal wastewater by an AnMBR was evaluated after an extended startup phase using only synthetic wastewater. Antibiotic resistance genes (ARGs) associated with sulfonamides, tetracyclines, and β-lactams were quantified, and effluent microbial community progression was analyzed. Results indicated that the AnMBR effluent inherently harbored all targeted ARGs prior to the introduction of real wastewater (104–109 copies/100 mL effluent). sul1, sul2, and intI1 genes were notably higher initially than other genes and markedly increased after the system was transitioned to municipal wastewater. Although potentially pathogenic bacteria made up over 20% relative abundance of the influent, AnMBR effluents showed a marginalization of these groups as their microbial communities more closely resembled the tightly bound layer of membrane biofilms. This work highlights the need for emerging treatment systems to be evaluated on a basis that incorporates the differentiation of system-associated ARGs and assesses their environmental transmission potential within the effluent communities.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Water Reuse and Desalination
Journal of Water Reuse and Desalination ENGINEERING, ENVIRONMENTAL-WATER RESOURCES
CiteScore
4.30
自引率
0.00%
发文量
23
审稿时长
16 weeks
期刊介绍: Journal of Water Reuse and Desalination publishes refereed review articles, theoretical and experimental research papers, new findings and issues of unplanned and planned reuse. The journal welcomes contributions from developing and developed countries.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信