犹大蒿分离纯化工艺优化其抗氧化和抗菌活性。Ahaggar（阿尔及利亚干旱地区）撒哈拉粗提物的UPLC-ESI-MS/MS级分分析

Q2 Pharmacology, Toxicology and Pharmaceutics

Current Bioactive Compounds Pub Date : 2023-07-18 DOI:10.2174/1573407219666230718125630

R. Kebbab, Aida basseddik, Walid Boussebaa, E. Nabti, K. Houali

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引用次数: 0

摘要

近年来，药用植物因寻找新的生物活性化合物而受到广泛关注。在这方面，我们一直对犹大蒿亚种感兴趣。撒哈拉种，被阿哈格尔的图阿雷格人广泛用于植物治疗。本研究旨在评估和优化该植物的生物活性，以评估其生物活性化合物的价值。为此，用甲醇（70%）进行萃取，然后使用极性增加的溶剂进行液-液分馏：乙醚、乙酸乙酯、正丁醇和水。除了对组分进行UPLC-ESI-MS/MS分析外，我们还实现了酚类、黄酮类化合物的分析以及抗氧化和抗菌活性的评估。还原效果与溶剂极性成正比。粗提取物的还原力最好（17.55±3.06µg/mL），酚类和类黄酮含量分别比其组分更好（20.35±0.5 mgGAE/gTDM）和（10.35±0.56 mgGAE/g TDM）。DPPH（2,2-二苯基-1-苦丁酰肼基）自由基清除测定显示，乙酸乙酯组分最具活性，IC50值最低（抑制浓度为DPPH的50%）（41.43±0.24µg/ml），其次是正丁醇（58.53±0.20µg/ml）、乙醚（135.07±6.18µg/ml，氢乙醇提取物的IC50值为114.05±3.37µg/mL。除肺炎克雷伯菌具有耐药性外，所有临床多耐药菌均观察到抗菌效果。观察到最重要的作用是乙酸乙酯部分对抗真菌菌株白色念珠菌。检测到了各种酚酸和黄酮类化合物（黄酮、黄酮醇、黄烷酮），这些物质可能与这些生物活性有关。我们可以得出结论，用极性增加的溶剂进行液-液萃取在优化这种植物的生物活性方面发挥着重要作用，这种植物含有多酚，因此可以作为天然抗氧化剂和抗菌剂的来源。

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Optimization of Antioxidant and Antimicrobial Activities by Fractionation of Artemisia judaica subsp. sahariensis Crude Extract from Ahaggar (Algerian Arid Region): UPLC-ESI-MS/MS Analysis of Fractions

In recent years, medicinal plants have received considerable attention due to the search for novel bioactive compounds. In this optic, we have been interested in Artemisia judaica subsp. Sahariensis, a Saharan species widely used in phytotherapy by the Tuaregs of the Ahaggar. This study aims to evaluate and optimize the biological activities of this plant in order to valorize its bioactive compounds. For this purpose, an extraction with methanol (70%) was carried out, then a liquid-liquid fractionation, using solvents with increasing polarity: diethyl ether, ethyl acetate, n-butanol and water. We realized an analysis of phenols, flavonoids and evaluation of antioxidant and antimicrobial activities in addition to the UPLC-ESI-MS/MS analysis of the fractions. The reducing effect was proportional to the solvent polarity. The crude extract gave the best reducing power (17.55±3.06µg/mL), better phenols and flavonoids contents (20.35±0.5 mgGAE/gTDM), (10.35±0.56 mgGAE/gTDM) respectively compared to its fractions. The DPPH (2,2-Diphenyl-1-Picrylhydrazyl) radical scavenging assay showed that the ethyl acetate fraction was the most active with the lowest IC50 value (inhibitory concentration to 50% of DPPH) (41.43±0.24 µg/ml) followed by n-butanol (58.53±0.20 µg/mL), diethyl ether (135.07±6.18 µg/mL) and aqueous (226.41±1.51 µg/mL) fractions (p<0.0001). Moreover, hydromethanolic extract gave an IC50 value of 114.05±3.37 µg/mL. The antimicrobial effect was observed on all clinical multi-resistant bacteria tested except Klebsiella pneumoniae, which was resistant. The most important effect was observed by the ethyl acetate fraction against the fungal strain Candida albicans. Various phenolic acids and flavonoids (flavones, flavonols, flavanones) were detected and could be responsible for these bioactivities. We can conclude that liquid-liquid extraction with solvents of increasing polarity plays a major role in optimizing the biological activity of this plant, which contains polyphenols and can therefore be valued as a source of natural antioxidants and antimicrobials.

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来源期刊

Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.90

自引率

0.00%

发文量

112

期刊介绍： The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.